Recombinant Rhodobacter sphaeroides L-alanine exporter AlaE (alaE)
Description
Introduction to Recombinant Rhodobacter sphaeroides L-Alanine Exporter AlaE (alaE)
The recombinant Rhodobacter sphaeroides L-alanine exporter AlaE (alaE) is a protein expressed in Escherichia coli and derived from the bacterium Rhodobacter sphaeroides. This protein is specifically designed to export L-alanine, an amino acid crucial for various cellular processes. The recombinant form of AlaE is engineered with an N-terminal His tag, facilitating its purification and identification.
Characteristics of Recombinant AlaE
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Protein Details: The recombinant AlaE protein consists of 135 amino acids (1-135aa) and is fused with an N-terminal His tag for easy purification and detection .
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Expression Host: It is expressed in E. coli, a common host for recombinant protein production due to its well-understood genetics and efficient expression systems .
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Purity and Storage: The protein is available in a lyophilized form with a purity of greater than 90% as determined by SDS-PAGE. It should be stored at -20°C or -80°C to maintain stability .
Function and Role of AlaE
AlaE functions as an exporter of L-alanine, helping to regulate intracellular levels of this amino acid. In bacteria like Escherichia coli, L-alanine exporters play a critical role in preventing toxic accumulation of L-alanine and its derivatives, which can inhibit cell growth .
Research Findings
While specific research on the Rhodobacter sphaeroides AlaE is limited, studies on similar L-alanine exporters in other bacteria provide valuable insights. For instance, the AlaE exporter in E. coli is crucial for maintaining intracellular L-alanine homeostasis and acts as a safety valve under conditions of high L-alanine levels .
Comparison with Other L-Alanine Exporters
Product Specs
Note: While we prioritize shipping the format currently in stock, please specify your format preference during order placement for customized preparation.
Note: All proteins are shipped with standard blue ice packs. Dry ice shipping requires advance notification and incurs additional charges.
The tag type is determined during production. If you require a specific tag, please inform us, and we will prioritize its development.
Target Background
KEGG: rsp:RSP_3651
STRING: 272943.RSP_3651
