Recombinant Rhodococcus erythropolis Sec-independent protein translocase protein TatA (tatA), partial

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Cat. No.

BT35893

Source

Yeast

Synonyms

tatA; Sec-independent protein translocase protein TatA

Purity

>85% (SDS-PAGE)

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Cat. No.

BT35893

Source

E.coli

Synonyms

tatA; Sec-independent protein translocase protein TatA

Purity

>85% (SDS-PAGE)

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Cat. No.

BT35893

Source

E.coli

Synonyms

tatA; Sec-independent protein translocase protein TatA

Purity

>85% (SDS-PAGE)

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Cat. No.

BT35893

Source

Baculovirus

Synonyms

tatA; Sec-independent protein translocase protein TatA

Purity

>85% (SDS-PAGE)

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Cat. No.

BT35893

Source

Mammalian cell

Synonyms

tatA; Sec-independent protein translocase protein TatA

Purity

>85% (SDS-PAGE)

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Description

Introduction to Recombinant Rhodococcus erythropolis TatA Protein

The Twin-arginine translocation (Tat) system is a Sec-independent protein transport pathway that enables the translocation of folded proteins across the cytoplasmic membrane in bacteria. In Rhodococcus erythropolis, the TatA protein is a critical component of this system, forming part of the translocase complex responsible for substrate recognition and membrane channel assembly . The recombinant "partial" TatA protein refers to a truncated form of this protein, often engineered for functional studies or biotechnological applications .

Tat System Composition

The Tat system in Rhodococcus spp. comprises three core proteins:

Protein	Role	Key Features
TatA	Channel formation	Single transmembrane helix, cytoplasmic domain, oligomerizes to form substrate-specific pores .
TatB	Substrate receptor	Binds twin-arginine signal peptides; collaborates with TatC .
TatC	Signal sequence recognition	Six transmembrane helices; essential for substrate targeting .

TatA Domains and Mechanism

Transmembrane domain: Anchors TatA to the cytoplasmic membrane.
Cytoplasmic amphipathic helix: Facilitates oligomerization and pore formation .
Proton-motive force (PMF) dependence: TatA-mediated translocation is driven by PMF rather than ATP hydrolysis .

Plasmid Vectors for Rhodococcus

Vector	Features	Expression Yield	Key Applications
pTipQC2	Thiostrepton-inducible promoter (P tipA), hexahistidine tag	Up to 10 mg/L culture	Heterologous protein production, co-expression studies .
pNit	Constitutive promoter	Variable	High-throughput screening .

Cloning and Purification

The TatA gene (tatA) is amplified via PCR and cloned into Rhodococcus-E. coli shuttle vectors (e.g., pTipQC2) .
Recombinant TatA is typically purified using nickel-affinity chromatography due to its hexahistidine tag .

Role in Lignin Transformation

The Tat system in Rhodococcus facilitates the secretion of lignin-modifying enzymes (e.g., peroxidases).
Recombinant TatA has been studied in R. erythropolis strains engineered for lignin valorization .

Biophysical Properties

Channel activity: Organic solvent extracts of R. erythropolis cell walls containing TatA exhibit ion channel activity in lipid bilayers .
Oligomerization: TatA forms dynamic pores sized according to substrate dimensions .

Comparative Analysis: Tat vs. Sec Pathways

Feature	Tat System	Sec System
Substrate state	Folded proteins	Unfolded polypeptides
Energy source	Proton-motive force	ATP (via SecA)
Signal peptide	Twin-arginine motif (RRXFLK)	Hydrophobic α-helix

Challenges and Future Directions

Low solubility: Truncated TatA variants may require co-expression with chaperones like GroEL/DnaK .
Industrial potential: Engineering TatA for enhanced secretion of biofuels or bioplastics in Rhodococcus .

Product Specs

Form

Lyophilized powder. We will ship the format we have in stock. If you have special format requirements, please note them when ordering.

Lead Time

Delivery time varies by purchasing method and location. Consult local distributors for specific delivery times. All proteins are shipped with blue ice packs by default. Requesting dry ice will incur extra fees and requires advance notice.

Notes

Avoid repeated freezing and thawing. Working aliquots can be stored at 4°C for up to one week.

Reconstitution

Briefly centrifuge the vial before opening. Reconstitute the protein in sterile deionized water to 0.1-1.0 mg/mL. Add 5-50% glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. Our default final glycerol concentration is 50%.

Shelf Life

Shelf life depends on storage conditions, buffer ingredients, storage temperature, and protein stability. Liquid form is generally stable for 6 months at -20°C/-80°C. Lyophilized form is generally stable for 12 months at -20°C/-80°C.

Storage Condition

Store at -20°C/-80°C upon receipt. Aliquot for multiple uses. Avoid repeated freeze-thaw cycles.

Tag Info

The tag type will be determined during production. If you require a specific tag, please inform us and we will prioritize its development.

Synonyms

tatA; Sec-independent protein translocase protein TatA

Buffer Before Lyophilization

Tris/PBS-based buffer, 6% Trehalose.

Datasheet

Please contact us to get it.

Protein Length

Partial

Purity

>85% (SDS-PAGE)

Species

Rhodococcus erythropolis (Arthrobacter picolinophilus)

Target Names

tatA

Uniprot No.

P72267

Target Background

Function

Part of the twin-arginine translocation (Tat) system, which transports large folded proteins containing a twin-arginine motif in their signal peptide across membranes. TatA may form the protein-conducting channel of the Tat system.

Protein Families

TatA/E family

Subcellular Location

Cell membrane; Single-pass membrane protein.

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Recombinant Rhodococcus erythropolis Sec-independent protein translocase protein TatA (tatA), partial

Description

Introduction to Recombinant Rhodococcus erythropolis TatA Protein

Tat System Composition

TatA Domains and Mechanism

Plasmid Vectors for Rhodococcus

Cloning and Purification

Role in Lignin Transformation

Biophysical Properties

Comparative Analysis: Tat vs. Sec Pathways

Challenges and Future Directions

Product Specs

Target Background

Quick Inquiry

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