Recombinant Rhodopirellula baltica 60 kDa chaperonin 2 (groL2), partial
Description
Rhodopirellula baltica
Rhodopirellula baltica is a marine bacterium known for its unique cellular structures and metabolic capabilities . It belongs to the Planctomycetes-Verrucomicrobia-Chlamydiae (PVC) superphylum and possesses a number of interesting features, including the ability to respond to environmental changes .
Chaperonins and GroL2
Chaperonins are a class of proteins that assist in the proper folding of other proteins, preventing aggregation and ensuring correct functionality . They are essential for cell survival, particularly under stress conditions. GroL2 is a specific type of chaperonin, a 60 kDa protein found in R. baltica .
Recombinant Production
Recombinant GroL2, partial, is produced through genetic engineering techniques, where the gene encoding a portion of the GroL2 protein is inserted into a host organism (e.g., Escherichia coli) or baculovirus system . The host organism then produces the protein, which is subsequently isolated and purified. The term "partial" indicates that the produced protein is not the full-length GroL2 but a fragment of it.
Function and Significance
Chaperonins like GroL2 are crucial for protein homeostasis within the cell. They are often upregulated under stress conditions, such as nutrient limitation, to maintain the correct folding of proteins and prevent aggregation .
R. baltica cells adapt metabolically to nutrient limitation by inducing chaperones .
Applications and Research
Studying recombinant GroL2, partial, can provide insights into:
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The structure and function of chaperonins.
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Potential biotechnological applications, such as protein folding and drug discovery.
Data Table: Gene Expression Studies in R. baltica
| Number of regulated genes | 62 h vs. 44 h | 82 h vs. 62 h | 96 h vs. 82 h | 240 h vs. 82 h |
|---|---|---|---|---|
| Total (%) * | 149 (2%) | 90 (1%) | 235 (3%) | 863 (12%) |
| Encoding hypothetical proteins (%) ** | 84 (56%) | 40 (44%) | 139 (59%) | 499 (58%) |
* (%) relative to the total number of 7325 open reading frames (ORFs) annotated in R. baltica genome.
** (%) relative to the total number of regulated genes.
Product Specs
Note: While we prioritize shipping the format currently in stock, please specify your format preference during order placement for customized preparation.
Note: All proteins are shipped with standard blue ice packs. Dry ice shipping requires prior arrangement and incurs additional charges.
The tag type is determined during production. If you require a specific tag, please inform us, and we will prioritize its development.
Target Background
KEGG: rba:RB8970
STRING: 243090.RB8970
