Recombinant Rhodopirellula baltica UPF0235 protein RB8260 (RB8260)
Description
Role in Rhodopirellula baltica
While RB8260’s exact function remains uncharacterized, genomic studies of R. baltica SH1 provide insights:
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Stress Adaptation: During stationary phase, R. baltica upregulates genes for cell wall modification, stress proteins (e.g., chaperones, thioredoxin), and genome rearrangement enzymes . Though RB8260 is not explicitly mentioned, its expression may align with these stress-response mechanisms.
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Biotechnological Potential: R. baltica harbors unique sulfatases and C1-metabolism genes, but RB8260 belongs to a distinct functional cluster .
Transcriptomic Data
A growth-phase analysis of R. baltica revealed dynamic gene regulation:
| Growth Phase | Regulated Genes | Hypothetical Proteins (%) |
|---|---|---|
| Mid-exponential (62 h) | 149 | 56% |
| Stationary (240 h) | 863 | 58% |
Hypothetical proteins like RB8260 constitute >50% of regulated genes, suggesting roles in metabolic adaptation or structural changes .
Research Applications
RB8260 is utilized in:
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Structural Studies: Full-length production enables crystallography or NMR analysis.
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Functional Annotation: As part of the UPF0235 family, it aids in deciphering conserved domains in Planctomycetes.
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Biochemical Assays: Available in multiple expression systems (e.g., biotinylated versions for pull-down assays) .
Limitations and Future Directions
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Uncharacterized Function: No direct experimental data link RB8260 to specific pathways.
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Expression Variability: Tagging and host systems (Yeast vs. Baculovirus) may influence protein behavior.
Comparative Product Availability
| Product Code | Source | Features |
|---|---|---|
| CSB-YP756144RDR | Yeast | Native-like post-translational modifications |
| CSB-BP756144RDR | Baculovirus | High-yield production |
| CSB-EP756144RDR-B | E. coli | AviTag-biotinylated for affinity assays |
Product Specs
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