Recombinant Rickettsia typhi Succinate dehydrogenase hydrophobic membrane anchor subunit (sdhD)
Description
Introduction to Recombinant Rickettsia typhi Succinate Dehydrogenase Hydrophobic Membrane Anchor Subunit (sdhD)
The Recombinant Rickettsia typhi Succinate dehydrogenase hydrophobic membrane anchor subunit (sdhD) is a crucial component of the succinate dehydrogenase (SDH) enzyme complex. This complex plays a pivotal role in the electron transport chain and is essential for cellular respiration. The sdhD subunit, specifically, serves as a hydrophobic membrane anchor, ensuring the proper integration of the SDH complex into the cell membrane.
Function and Importance of sdhD
The succinate dehydrogenase complex, including the sdhD subunit, is vital for converting succinate to fumarate during the citric acid cycle (also known as the Krebs cycle or tricarboxylic acid cycle). This process not only generates energy but also contributes to the electron transport chain, producing ATP. The sdhD subunit is crucial for anchoring the complex to the inner mitochondrial membrane in eukaryotic cells, although in prokaryotes like Rickettsia typhi, it anchors the complex to the cell membrane.
Research and Development of Recombinant sdhD
Recombinant Rickettsia typhi sdhD proteins are produced using various expression systems, including E. coli, yeast, baculovirus, and mammalian cells. These proteins are often used in research to study the structure and function of the SDH complex and its role in cellular metabolism. The recombinant full-length Rickettsia typhi sdhD protein, for example, is available with an N-terminal His tag, facilitating purification and detection .
Table 1: Expression Systems for Recombinant Rickettsia typhi sdhD
| Expression System | Description |
|---|---|
| E. coli | Commonly used for high-yield protein production, suitable for structural and functional studies. |
| Yeast | Offers a more eukaryotic-like environment, beneficial for studying protein modifications. |
| Baculovirus | Utilizes insect cells, ideal for producing complex proteins with proper folding. |
| Mammalian Cells | Provides a native eukaryotic environment, useful for studying protein interactions and modifications. |
Applications and Research Findings
The recombinant sdhD subunit is valuable in biochemical and biophysical studies aimed at understanding the assembly and function of the SDH complex. It can also serve as a tool for investigating the pathogenic mechanisms of Rickettsia typhi, which causes murine typhus. Studies involving recombinant proteins like sdhD can help elucidate how these pathogens interact with host cells and evade immune responses.
Table 2: Key Features of Recombinant Rickettsia typhi sdhD
| Feature | Description |
|---|---|
| Protein Length | Partial or full-length (1-125 amino acids) |
| Purity | >85% (SDS-PAGE) |
| Species | Rickettsia typhi (strain ATCC VR-144 / Wilmington) |
| Uniprot No. | Q68XP0 |
| Subcellular Location | Cell inner membrane; multi-pass membrane protein |
Product Specs
Note: While we prioritize shipping the format currently in stock, please specify your format preference in order notes for customized fulfillment.
Note: All proteins are shipped with standard blue ice packs. Dry ice shipping requires prior arrangement and incurs additional charges.
The tag type will be determined during production. If you require a specific tag, please inform us, and we will prioritize its implementation.
Target Background
Membrane-anchoring subunit of succinate dehydrogenase (SDH).
KEGG: rty:RT0116
STRING: 257363.RT0116
