Recombinant Saccharomyces cerevisiae Eukaryotic translation initiation factor 5A-1 (HYP2)
Description
Overview of Recombinant HYP2
HYP2, encoded by the TIF51A gene in S. cerevisiae, is a hypusine-containing translation factor essential for cell viability . Recombinant HYP2 is produced via heterologous expression systems (e.g., E. coli or yeast) with affinity tags (e.g., His-SUMO) for purification . It spans amino acids 2–157 of the native protein and has a molecular weight of ~33 kDa .
Translation Elongation
HYP2 facilitates peptide bond formation, particularly for polyproline motifs that stall ribosomes . Key findings:
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Depletion of HYP2 reduces polysome formation and delays ribosomal transit .
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Recombinant HYP2 increases tripeptide synthesis rates by ~2–3 fold in vitro .
Stress Response
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Acetic Acid Tolerance: Overexpression of HYP2 enhances yeast survival under acetic acid stress by upregulating transcription factor Ume6p and its targets (BEM4, BUD21, IME4) .
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Thermal/Osmotic Stress: HYP2 activity correlates with stress adaptation, likely via mRNA stability and translation efficiency .
Cell Cycle and Morphogenesis
Mechanistic Studies
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Ribosome Profiling: Used to identify HYP2-dependent stalling sequences (e.g., polyproline tracts) .
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Mutational Analysis: Hypusine-deficient mutants (e.g., Lys51 substitutions) reveal functional requirements .
Industrial Biotechnology
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Engineered HYP2 overexpression improves yeast tolerance to fermentation inhibitors (e.g., acetic acid) .
Key Research Findings
Current Challenges and Future Directions
Product Specs
Target Background
KEGG: sce:YEL034W
STRING: 4932.YEL034W
