Recombinant Saccharomyces cerevisiae H/ACA ribonucleoprotein complex subunit 2 (NHP2)

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Cat. No.

BT32192

Source

Yeast

Synonyms

NHP2; YDL208W; D1045; H/ACA ribonucleoprotein complex subunit NHP2; H/ACA snoRNP protein NHP2; High mobility group-like nuclear protein 2

Purity

>85% (SDS-PAGE)

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Cat. No.

BT32192

Source

E.coli

Synonyms

NHP2; YDL208W; D1045; H/ACA ribonucleoprotein complex subunit NHP2; H/ACA snoRNP protein NHP2; High mobility group-like nuclear protein 2

Purity

>85% (SDS-PAGE)

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Cat. No.

BT32192

Source

E.coli

Synonyms

NHP2; YDL208W; D1045; H/ACA ribonucleoprotein complex subunit NHP2; H/ACA snoRNP protein NHP2; High mobility group-like nuclear protein 2

Purity

>85% (SDS-PAGE)

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Cat. No.

BT32192

Source

Baculovirus

Synonyms

NHP2; YDL208W; D1045; H/ACA ribonucleoprotein complex subunit NHP2; H/ACA snoRNP protein NHP2; High mobility group-like nuclear protein 2

Purity

>85% (SDS-PAGE)

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Cat. No.

BT32192

Source

Mammalian cell

Synonyms

NHP2; YDL208W; D1045; H/ACA ribonucleoprotein complex subunit NHP2; H/ACA snoRNP protein NHP2; High mobility group-like nuclear protein 2

Purity

>85% (SDS-PAGE)

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Description

Molecular and Functional Overview

Nhp2 (Non-Histone Protein 2) is a conserved 22 kDa protein encoded by the YDL208W gene in S. cerevisiae . It forms part of the H/ACA RNP complex alongside Cbf5 (dyskerin), Gar1, and Nop10 . Key features include:

Property	Details
Amino Acid Sequence	IKADPDGPEAQAEACSGERTYQELLVNQNPIAQPLASRRLTRKLYKCIKKAVKQKQIRRGVKEVQKFVNKGE
Domains	L7Ae RNA-binding domain, facilitating interactions with H/ACA snoRNAs
Function	Stabilizes snoRNA-RNP interactions; mediates rRNA pseudouridylation

Recombinant Production Systems

Recombinant Nhp2 is commercially available in multiple expression platforms, enabling flexibility for structural and functional studies:

Expression System	Source	Tag/Purification Method	Applications
E. coli	Bio-Techne	N-terminal His6ABP tag, IMAC purified	Antibody competition assays
Yeast	Cusabio	Native conformation	Structural studies
Baculovirus	Cusabio	High-yield eukaryotic expression	Interaction assays (e.g., pull-downs)
Mammalian cells	Cusabio	Post-translational modifications	Functional genomics

Key Notes:

E. coli-derived Nhp2 is widely used due to cost-effectiveness, but lacks eukaryotic post-translational modifications .
Yeast-expressed Nhp2 retains native folding, critical for studying RNP assembly .

Ribosome Biogenesis Studies

Recombinant Nhp2 has been used to reconstitute H/ACA RNPs in vitro, demonstrating its role in co-transcriptional rRNA folding .
Loss of Nhp2 disrupts 18S rRNA processing intermediates (32S, 23S), highlighting its necessity in small ribosomal subunit maturation .

Disease Relevance

Mutations in human NHP2 homologs are linked to dyskeratosis congenita, a ribosomopathy causing bone marrow failure .
Yeast Nhp2 mutants show hypersensitivity to translational inhibitors (e.g., paromomycin), underscoring its role in ribosome fidelity .

Future Directions

Structural Dynamics: Resolving conformational changes during RNP assembly using yeast-derived Nhp2 .
Therapeutic Targeting: Screening small molecules against recombinant Nhp2 to address ribosomopathies .

Product Specs

Form

Lyophilized powder. We will ship the in-stock format unless you specify a format preference when ordering.

Lead Time

Delivery times vary by purchase method and location. Consult your local distributor for specifics. Proteins ship with blue ice packs by default; request dry ice in advance for an extra fee.

Notes

Avoid repeated freezing and thawing. Working aliquots are stable at 4°C for up to one week.

Reconstitution

Briefly centrifuge the vial before opening. Reconstitute protein in sterile deionized water to 0.1-1.0 mg/mL. Add 5-50% glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. The default final glycerol concentration is 50%.

Shelf Life

Shelf life depends on storage conditions, buffer, temperature, and protein stability. Liquid form: 6 months at -20°C/-80°C. Lyophilized form: 12 months at -20°C/-80°C.

Storage Condition

Store at -20°C/-80°C upon receipt. Aliquot for multiple uses. Avoid repeated freeze-thaw cycles.

Tag Info

The tag type is determined during manufacturing. Specify your preferred tag type, and we will prioritize its development.

Synonyms

NHP2; YDL208W; D1045; H/ACA ribonucleoprotein complex subunit NHP2; H/ACA snoRNP protein NHP2; High mobility group-like nuclear protein 2

Buffer Before Lyophilization

Tris/PBS-based buffer, 6% Trehalose.

Datasheet

Please contact us to get it.

Expression Region

1-156

Protein Length

full length protein

Purity

>85% (SDS-PAGE)

Species

Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)

Target Names

NHP2

Target Protein Sequence

MGKDNKEHKE SKESKTVDNY EARMPAVLPF AKPLASKKLN KKVLKTVKKA SKAKNVKRGV KEVVKALRKG EKGLVVIAGD ISPADVISHI PVLCEDHSVP YIFIPSKQDL GAAGATKRPT SVVFIVPGSN KKKDGKNKEE EYKESFNEVV KEVQAL

Uniprot No.

P32495

Target Background

Function

NHP2 is a non-catalytic component of the H/ACA snoRNP complex, which catalyzes rRNA pseudouridylation, essential for ribosome biogenesis. This involves isomerizing uridine so ribose attaches to C5 instead of N1. Pseudouridine ('psi') likely stabilizes rRNA conformation. The H/ACA snoRNP complex also pseudouridylates other RNAs, such as U2 snRNA at position 93. NHP2 is essential for growth and directly binds H/ACA snoRNAs.

Database Links

KEGG: sce:YDL208W

STRING: 4932.YDL208W

Protein Families

Eukaryotic ribosomal protein eL8 family

Subcellular Location

Nucleus, nucleolus.

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Recombinant Saccharomyces cerevisiae H/ACA ribonucleoprotein complex subunit 2 (NHP2)

Description

Molecular and Functional Overview

Recombinant Production Systems

Key Notes:

Ribosome Biogenesis Studies

Disease Relevance

Future Directions

Product Specs

Target Background

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