Recombinant Saccharomyces cerevisiae Origin recognition complex subunit 5 (ORC5)

Shipped with Ice Packs
In Stock
Cat. No.
BT43933
Source
Yeast
Synonyms
ORC5; YNL261W; N0834; Origin recognition complex subunit 5; Origin recognition complex 53 kDa subunit
Purity
>85% (SDS-PAGE)
Quick Inquiry
Cat. No.
BT43933
Source
E.coli
Synonyms
ORC5; YNL261W; N0834; Origin recognition complex subunit 5; Origin recognition complex 53 kDa subunit
Purity
>85% (SDS-PAGE)
Quick Inquiry
Cat. No.
BT43933
Source
E.coli
Synonyms
ORC5; YNL261W; N0834; Origin recognition complex subunit 5; Origin recognition complex 53 kDa subunit
Purity
>85% (SDS-PAGE)
Quick Inquiry
Cat. No.
BT43933
Source
Baculovirus
Synonyms
ORC5; YNL261W; N0834; Origin recognition complex subunit 5; Origin recognition complex 53 kDa subunit
Purity
>85% (SDS-PAGE)
Quick Inquiry
Cat. No.
BT43933
Source
Mammalian cell
Synonyms
ORC5; YNL261W; N0834; Origin recognition complex subunit 5; Origin recognition complex 53 kDa subunit
Purity
>85% (SDS-PAGE)
Quick Inquiry

Description

Biochemical Characterization

Recombinant ORC5 refers to the subunit expressed and purified in vitro using systems such as rabbit reticulocyte lysate, baculovirus-infected insect cells, or mammalian expression vectors . Key features include:

PropertyDescription
Molecular Weight~53 kDa (predicted)
ATP-BindingBinds ATP via Walker A/B motifs in its AAA+ domain
ConservationHighly conserved in AAA+ fold; diverges in N-terminal regions across species
Subunit InteractionsDirectly binds ORC4 and associates with ORC1-3 via ORC2/3 interfaces

ATP hydrolysis by ORC5 is not essential for yeast viability but contributes to ORC stability and DNA replication fidelity . Mutations in its Walker A motif (e.g., K118A) disrupt ATP binding, impairing pre-RC assembly and replication in humanized systems .

DNA Replication Initiation

  • ORC5 is indispensable for ORC’s high-affinity binding to Autonomously Replicating Sequences (ARS) .

  • ATP binding by ORC5 stabilizes ORC-DNA interactions, with mutations (e.g., GKT→GAT) reducing replication efficiency in Xenopus extracts .

  • In S. cerevisiae, ORC5 mutants defective in ATP binding (e.g., orc5-1) retain silencing functions but fail to initiate replication, highlighting separable roles in replication and chromatin regulation .

Subunit Assembly

  • ORC5 bridges ORC4 and ORC2/3 subcomplexes. Yeast two-hybrid assays confirm direct ORC4-ORC5 binding, dependent on ORC5’s ATPase activity .

  • Recombinant ORC1-5 subcomplexes lacking ORC6 retain DNA-binding and replication competence, underscoring ORC5’s structural centrality .

Key Mutational Studies

  • Walker A Motif Mutants: Disrupt ATPase activity, impairing chromatin loading and pre-RC formation in human ORC .

  • N-terminal Truncations: Reduce ORC stability but preserve DNA binding, suggesting a regulatory role for the N-terminus .

Expression Systems and Applications

Recombinant ORC5 is typically co-expressed with other ORC subunits for functional studies:

  • Baculovirus Systems: Yield ORC1-5 complexes with near-stoichiometric subunit ratios .

  • Mammalian Systems: Enable humanized ORC studies, revealing conserved ATPase mechanisms .

Unresolved Questions

  • How do ORC5’s ATPase activities coordinate with ORC1 and ORC4 during origin licensing?

  • What structural rearrangements occur in ORC5 during helicase loading?

Product Specs

Form
Lyophilized powder. We will preferentially ship the format we have in stock. If you have special format requirements, please note them when ordering.
Lead Time
Delivery times vary by purchasing method and location. Consult local distributors for specific delivery times. Proteins are shipped with blue ice packs by default. Request dry ice in advance for an extra fee.
Notes
Avoid repeated freeze-thaw cycles. Store working aliquots at 4°C for up to one week.
Reconstitution
Briefly centrifuge the vial before opening. Reconstitute protein in sterile deionized water to 0.1-1.0 mg/mL. Add 5-50% glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. Our default final glycerol concentration is 50%.
Shelf Life
Shelf life depends on storage conditions, buffer, temperature, and protein stability. Liquid form: 6 months at -20°C/-80°C. Lyophilized form: 12 months at -20°C/-80°C.
Storage Condition
Store at -20°C/-80°C upon receipt. Aliquot for multiple uses. Avoid repeated freeze-thaw cycles.
Tag Info
Tag type is determined during manufacturing. If you require a specific tag, please inform us and we will prioritize its development.
Synonyms
ORC5; YNL261W; N0834; Origin recognition complex subunit 5; Origin recognition complex 53 kDa subunit
Buffer Before Lyophilization
Tris/PBS-based buffer, 6% Trehalose.
Datasheet
Please contact us to get it.
Expression Region
1-479
Protein Length
full length protein
Purity
>85% (SDS-PAGE)
Species
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Target Names
ORC5
Target Protein Sequence
MNVTTPEVAF REYQTNCLAS YISADPDITP SNLILQGYSG TGKTYTLKKY FNANPNLHAV WLEPVELVSW KPLLQAIART VQYKLKTLYP NIPTTDYDPL QVEEPFLLVK TLHNIFVQYE SLQEKTCLFL ILDGFDSLQD LDAALFNKYI KLNELLPKDS KINIKFIYTM LETSFLQRYS THCIPTVMFP RYNVDEVSTI LVMSRCGELM EDSCLRKRII EEQITDCTDD QFQNVAANFI HLIVQAFHSY TGNDIFALND LIDFKWPKYV SRITKENIFE PLALYKSAIK LFLSTDDNLS ENGQGESAIT TNRDDLENSQ TYDLSIISKY LLIASYICSY LEPRYDASIF SRKTRIIQGR AAYGRRKKKE VNPRYLQPSL FAIERLLAIF QAIFPIQGKA ESGSLSALRE ESLMKANIEV FQNLSELHTL KLIATTMNKN IDYLSPKVRW KVNVPWEIIK EISESVHFNI SDYFSDIHE
Uniprot No.
P50874

Target Background

Function
ORC5 is part of the origin recognition complex (ORC), which binds to replication origins. It is involved in chromosome replication and mating type transcriptional silencing. ORC5 binds to the ARS consensus sequence (ACS) at replication origins and may mediate ATP-dependent ORC binding. It may also be involved in targeting substrates for the cullin-RING E3 ubiquitin-protein ligase complex RTT101(MMS1-ORC5).
Gene References Into Functions
1. Rrm3, through its Orc5-binding domain, restricts DNA synthesis, a function separate from its role in facilitating fork progression through replication blocks. (PMID: 27923055) 2. The interaction is mediated by the C-terminal region of Orc4p and the N-terminal region of Orc5p. (PMID: 17107343)
Database Links

KEGG: sce:YNL261W

STRING: 4932.YNL261W

Protein Families
ORC5 family
Subcellular Location
Nucleus.

Quick Inquiry

Personal Email Detected
Please use an institutional or corporate email address for inquiries. Personal email accounts ( such as Gmail, Yahoo, and Outlook) are not accepted. *

Category

Service

THE BIOTEK
THE BioTek is a global biotech company offering highly purified proteins for research in cancer, apoptosis, development, endocrinology, immunology, neuroscience, proteases, and stem cells.
  • Contact
  • Address: 1925 E Maple Ave, El Segundo, CA 90245 United States
  • Phone : +1 201-285-7826
  • Email : info@thebiotek.com
  • Hours : Mon.-Fri. 9:00 AM - 5:00 PM
© Copyright 2025 TheBiotek. All Rights Reserved.