Recombinant Saccharomyces cerevisiae Phosphoglucomutase-1 (PGM1), partial

Shipped with Ice Packs
In Stock

Product Specs

Form
Lyophilized powder. We may ship a different format if available, but will fulfill specific format requests upon order.
Lead Time
Delivery times vary by purchase method and location. Consult local distributors for specific delivery times. Proteins are shipped with blue ice packs by default; dry ice shipping requires prior arrangement and incurs extra fees.
Notes
Avoid repeated freezing and thawing. Store working aliquots at 4°C for up to one week.
Reconstitution
Briefly centrifuge the vial before opening. Reconstitute protein in sterile deionized water to 0.1-1.0 mg/mL. Add 5-50% glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. Our default final glycerol concentration is 50%.
Shelf Life
Shelf life depends on storage conditions, buffer components, temperature, and protein stability. Liquid form: generally 6 months at -20°C/-80°C. Lyophilized form: generally 12 months at -20°C/-80°C.
Storage Condition
Store at -20°C/-80°C upon receipt. Aliquot for multiple uses. Avoid repeated freeze-thaw cycles.
Tag Info
Tag type is determined during manufacturing. If you require a specific tag, please inform us and we will prioritize its development.
Synonyms
PGM1; YKL127W; Phosphoglucomutase 1; PGM 1; EC 5.4.2.2; Glucose phosphomutase 1
Buffer Before Lyophilization
Tris/PBS-based buffer, 6% Trehalose.
Datasheet
Please contact us to get it.
Protein Length
Partial
Purity
>85% (SDS-PAGE)
Species
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Target Names
PGM1
Uniprot No.

Target Background

Function
PGM1 is a minor phosphoglucomutase isozyme that converts glucose 1-phosphate and glucose 6-phosphate. It represents 10-20% of cellular phosphoglucomutase activity and is crucial for hexose metabolism. The forward reaction is essential for galactose metabolism, while the reverse reaction is essential for biosynthesis when using non-galactose carbon sources, facilitating UDP-glucose synthesis for oligosaccharides and trehalose production.
Gene References Into Functions
Elevated phosphoglucomutase activity allows for more efficient use of limited glucose-1-phosphate levels in the RAS2(val19) mutant. (PMID: 16292676)
Database Links

KEGG: sce:YKL127W

STRING: 4932.YKL127W

Protein Families
Phosphohexose mutase family
Subcellular Location
Cytoplasm.

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