Recombinant Saccharomyces cerevisiae Putative uncharacterized protein HUR1 (HUR1)
Description
Introduction to Recombinant Saccharomyces cerevisiae Putative Uncharacterized Protein HUR1 (HUR1)
Recombinant Saccharomyces cerevisiae Putative Uncharacterized Protein HUR1 (HUR1) is a bioengineered protein derived from the yeast gene HUR1 (UniProt ID: P45820). Originally identified in S. cerevisiae strain S288C , this protein is expressed in E. coli with an N-terminal His-tag for purification and functional studies . Despite its classification as "uncharacterized," HUR1 has been implicated in critical cellular processes, including DNA repair mechanisms .
DNA Repair Mechanisms
HUR1 deletion (specifically HUR1-A, a non-overlapping region with PMR1) reduces the efficiency of Non-Homologous End Joining (NHEJ), a key pathway for repairing double-strand breaks (DSBs) . Key findings include:
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Plasmid Repair Assays: Reduced repair efficiency for both blunt-end and overhang DSBs .
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Chromosomal Repair: Impaired DSB repair in chromosomal contexts .
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Genetic Interactions: Epistatic relationships with TPK1 and NEJ1, suggesting functional overlap in NHEJ pathways .
Additional Roles
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Homologous Recombination (HR): HUR1 may indirectly influence HR repair, though its role is less defined .
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Protein Interactions: Associates with PMR1 (calcium-transporting ATPase), AUA1 (ammonia regulation), and WWM1 (apoptosis regulation) .
Research Applications and Experimental Utilities
Common Uses
Experimental Considerations
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Reconstitution: Dissolve in deionized water (0.1–1.0 mg/mL) with 5–50% glycerol for long-term storage .
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Stability: Avoid repeated freeze-thaw cycles; store at -20°C/-80°C .
Protein Interaction Network
Predicted Partners
| Protein | Function | Interaction Score |
|---|---|---|
| PMR1 | Calcium/Mn²⁺ transport | 0.970 |
| AUA1 | Ammonia sensing | 0.713 |
| WWM1 | Apoptosis regulation | 0.604 |
| NAG1 | Cell wall biogenesis | 0.517 |
Functional Implications
HUR1’s interaction with PMR1 may link DNA repair to ion homeostasis, while associations with AUA1 and WWM1 suggest roles in stress responses .
Product Specs
Note: While we prioritize shipping the format currently in stock, we are happy to accommodate specific format requests. Please indicate your preference in the order notes, and we will prepare the product accordingly.
Note: Our standard shipping includes blue ice packs. If dry ice shipping is required, please inform us in advance, as additional fees will apply.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Target Background
- Using a Homologous Recombination (HR) specific plasmid-based DSB repair assay, we observed that deletion of HUR1-A influenced the efficiency of HR repair, suggesting that HUR1 might also play additional roles in other DNA repair pathways PMID: 28987344
KEGG: sce:YGL168W
STRING: 4932.YGL168W
