Recombinant Saccharomyces cerevisiae Putative uncharacterized protein YHR052W-A (YHR052W-A)
Description
Key Features:
Recombinant Production
Recombinant YHR052W-A is produced using E. coli expression systems. The protein is purified via affinity chromatography and supplied in a lyophilized Tris/PBS buffer with 6% trehalose (pH 8.0) . Storage recommendations include aliquoting to avoid repeated freeze-thaw cycles and maintaining temperatures at -20°C or -80°C .
Functional Insights
While native YHR052W-A is considered non-functional, recombinant studies suggest limited roles:
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Calcium Sensitivity Suppression: Overexpression suppresses Ca²⁺ sensitivity in mutants lacking inositol phosphorylceramide mannosyltransferases Csg1p and Csh1p .
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Membrane Localization: Predicted to be a single-pass membrane protein, though this remains unverified experimentally .
Research Applications
Recombinant YHR052W-A is primarily used in:
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Antibody Development: Rabbit polyclonal antibodies (e.g., MBS7151367) target this protein for ELISA and Western blot applications .
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Protein Interaction Studies: STRING database annotations highlight weak associations with proteins like YHR054W-A (a dubious ORF) and HOR7 (a hyperosmotic stress-responsive protein) .
Limitations and Controversies
The YHR052W-A gene’s dubious status complicates its study. UniProt and SGD emphasize that comparative sequence data and experimental evidence do not support a functional role in S. cerevisiae . Recombinant versions may thus serve as negative controls or tools for probing gene annotation accuracy.
Product Specs
Note: We prioritize shipping the format currently in stock. However, if you have specific format requirements, please indicate them when placing your order. We will fulfill your request if possible.
Note: All our proteins are shipped with standard blue ice packs. If you require dry ice shipping, please inform us in advance, as additional fees may apply.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
