Recombinant Saccharomyces cerevisiae Spore membrane assembly protein 2 (SMA2)
Description
Introduction to SMA2
SMA2 (YML066C) is a meiosis-specific protein essential for prospore membrane assembly during sporulation in Saccharomyces cerevisiae. It ensures proper bending and closure of the prospore membrane to encapsulate haploid nuclei post-meiosis .
Gene and Protein Overview
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Gene: SMA2 is located on chromosome XIII and regulated by the transcription factor Ndt80 during the middle sporulation phase .
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Protein: SMA2 is a 294-amino acid protein with no conserved domains but shares structural similarities with other prospore membrane components like Spo74 .
Recombinant Production
Key Activities
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Prospore Membrane Bending: SMA2 generates mechanical force to curve the prospore membrane, ensuring proper engulfment of haploid nuclei .
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Interaction Network: Collaborates with Spo21/Mpc70 and Spo74 to form the meiosis II outer plaque (MOP), a structure critical for membrane assembly .
Regulatory Mechanisms
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Transcriptional Control: Co-induced with mORC1 via a bidirectional promoter element activated by Ndt80 during meiosis .
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Post-Translational Modifications: Requires phosphorylation by kinases such as Ime2 for functional activation .
Experimental Use Cases
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Sporulation Studies: Used to investigate prospore membrane dynamics and genetic interactions in yeast sporulation mutants .
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Protein Interaction Mapping: Identified 223 interactors via BioGRID, including Spo74 and components of the spindle pole body .
Limitations
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Partial-Length Protein: The recombinant product lacks full-length sequence coverage, potentially omitting functional domains .
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Species Specificity: Functionally distinct from human SMN proteins involved in spinal muscular atrophy .
Key Research Findings
Future Directions
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Full-Length Protein Synthesis: Addressing current limitations in recombinant SMA2 coverage to enable structural studies.
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Mechanistic Studies: Elucidating how SMA2-generated forces are transmitted to the prospore membrane.
Product Specs
Note: We will prioritize shipping the format currently in stock. However, if you have specific format requirements, please indicate them in your order notes. We will fulfill your request to the best of our ability.
Note: All our proteins are shipped with standard blue ice packs by default. If you require dry ice shipping, please inform us in advance. Additional fees may apply.
Generally, liquid forms have a shelf life of 6 months at -20°C/-80°C. Lyophilized forms have a shelf life of 12 months at -20°C/-80°C.
