Recombinant Saccharum hybrid ATP synthase subunit a, chloroplastic (atpI)
Description
Functional Role in ATP Synthase
Subunit a (atpI) is integral to the F<sub>O</sub> sector’s rotary mechanism:
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Proton Translocation: Collaborates with the c-subunit ring (c<sub>10</sub>–c<sub>14</sub>) to drive rotation via H<sup>+</sup> gradient .
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Structural Stabilization: Anchors the c-ring to prevent ion leakage and maintain coupling efficiency .
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Na<sup>+</sup> Specificity: In related archaeal ATP synthases, subunit a homologs enable Na<sup>+</sup>-dependent activity, suggesting evolutionary conservation of ion selectivity .
3.1. Cooperativity in c-Subunit Rotation
Studies on Bacillus PS3 ATP synthase revealed that mutations in adjacent c-subunits (e.g., cE56D) reduce ATP synthesis efficiency. This highlights functional coupling between subunit a and the c-ring, where proton transfer kinetics depend on cooperative interactions .
3.3. Role in Thermodynamic Efficiency
Chloroplast ATP synthases operate near thermodynamic limits (Δψ ≈ 90–150 mV). Subunit a’s proton channel geometry optimizes energy transduction, enabling ATP synthesis even at low membrane potentials .
Applications in Research
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Mechanistic Studies: Used to dissect proton-driven rotation in plant ATP synthases .
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Bioengineering: Template for modifying ion specificity (e.g., H<sup>+</sup> vs. Na<sup>+</sup>) to enhance crop photosynthetic efficiency .
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Drug Development: Screening platform for ATP synthase inhibitors targeting F<sub>O</sub> sector dysfunction .
Product Specs
Note: We prioritize shipping the format currently in stock. However, if you have specific format requirements, please indicate them during order placement. We will strive to accommodate your request.
Note: All our proteins are shipped with standard blue ice packs. If dry ice shipping is required, please inform us in advance, as additional fees will apply.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
