Recombinant Saguinus oedipus Ras-related protein Ral-A (RALA)

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Cat. No.

BT45257

Source

Yeast

Synonyms

RALA; RAL; Ras-related protein Ral-A

Purity

>85% (SDS-PAGE)

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Cat. No.

BT45257

Source

E.coli

Synonyms

RALA; RAL; Ras-related protein Ral-A

Purity

>85% (SDS-PAGE)

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Cat. No.

BT45257

Source

E.coli

Synonyms

RALA; RAL; Ras-related protein Ral-A

Purity

>85% (SDS-PAGE)

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Cat. No.

BT45257

Source

Baculovirus

Synonyms

RALA; RAL; Ras-related protein Ral-A

Purity

>85% (SDS-PAGE)

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Cat. No.

BT45257

Source

Mammalian cell

Synonyms

RALA; RAL; Ras-related protein Ral-A

Purity

>85% (SDS-PAGE)

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Description

Overview of Human RALA

Ras-related protein Ral-A (RALA) is a small GTPase belonging to the Ras superfamily. It functions as a molecular switch, cycling between inactive GDP-bound and active GTP-bound states to regulate processes such as vesicle trafficking, mitochondrial fission, and cell division .

Key Functional Domains:

Domain/Region	Characteristics
GTP-binding domain	Binds GTP/GDP; mediates activation/inactivation cycles
Switch I/II regions	Interact with downstream effectors (e.g., RALBP1, SEC5, EXO84)
Hypervariable C-tail	Determines subcellular localization; phosphorylation sites (e.g., Ser194 in RALA)

Role in Cellular Processes

RALA interacts with diverse effectors to coordinate critical functions:

Effector Interactions and Outcomes:

Effector	Binding Region	Biological Process
RALBP1	Switch I/II	Mitochondrial fission, endocytosis
SEC5/EXO84	Switch I	Exocyst assembly, vesicle trafficking
PLD1	N-terminal	Phospholipase D activation

Cancer Associations:

Anchorage-independent growth: RALA promotes tumorigenesis in bladder, prostate, and pancreatic cancers .
Expression correlation: High RALA levels correlate with advanced cancer stages and poor prognosis .

Pharmacological Targeting:

Approach	Mechanism	Status
RALGAP activation	Enhances GTP hydrolysis to inactivate RAL	Preclinical
Effector inhibition	Disrupts RALBP1 or exocyst interactions	Experimental

Recombinant RALA Production (General Workflow)

While no data exists for Saguinus oedipus RALA, human recombinant RALA is typically generated via:

Gene Cloning: Isolate RALA cDNA and insert into expression vectors.
Expression Systems: Use bacterial (E. coli) or mammalian (HEK293) systems.
Purification: Affinity chromatography (e.g., GST-tagged RALA).
Functional Assays: GTPase activity measured via malachite green assay .

Research Gaps and Recommendations

Species-specific data: No studies on Saguinus oedipus RALA exist; comparative genomics or de novo sequencing would be required.
Functional studies: Prioritize cross-species homology modeling to infer tamarin RALA behavior.

Product Specs

Form

Lyophilized powder. We will ship the available format, but if you have special requirements, please note them when ordering, and we will fulfill your request.

Lead Time

Delivery times vary based on purchasing method and location. Consult your local distributor for specific delivery times. All proteins are shipped with standard blue ice packs. Requesting dry ice will incur extra fees; please inform us in advance.

Notes

Avoid repeated freeze-thaw cycles. Working aliquots are stable at 4°C for up to one week.

Reconstitution

Briefly centrifuge the vial before opening. Reconstitute the protein in sterile deionized water to 0.1-1.0 mg/mL. Add 5-50% glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. Our default final glycerol concentration is 50%.

Shelf Life

Shelf life depends on storage conditions, buffer components, storage temperature, and protein stability. Liquid form: 6 months at -20°C/-80°C. Lyophilized form: 12 months at -20°C/-80°C.

Storage Condition

Store at -20°C/-80°C upon receipt. Aliquot for multiple uses. Avoid repeated freeze-thaw cycles.

Tag Info

The tag type is determined during manufacturing. If you require a specific tag, please inform us, and we will prioritize its development.

Synonyms

RALA; RAL; Ras-related protein Ral-A

Buffer Before Lyophilization

Tris/PBS-based buffer, 6% Trehalose.

Datasheet

Please contact us to get it.

Expression Region

1-203

Protein Length

full length protein

Purity

>85% (SDS-PAGE)

Species

Saguinus oedipus (Cotton-top tamarin)

Target Names

RALA

Target Protein Sequence

MAANKPKGQN SLALHKVIMV GSGGVGKSAL TLQFMYDEFV EDYEPTKADS YRKKVVLDGE EVQIDILDTA GQEDYAAIRD NYFRSGEGFL CVFSITEMES FAATADFREQ ILRVKEDENV PFLLVGNKSD LEDKRQVSVE EAKNRADQWN VNYVETSAKT RANVDKVFFD LMREIRARKM EDSKEKNGKK KRKSLAKRIR ERC

Uniprot No.

P63320

Target Background

Function

RALA is a multifunctional GTPase involved in various cellular processes, including gene expression, cell migration, cell proliferation, oncogenic transformation, and membrane trafficking. It interacts with distinct downstream effectors. RALA acts as a GTP sensor for dense core vesicle exocytosis. The RALA-exocyst complex regulates integrin-dependent membrane raft exocytosis and growth signaling. It's a key regulator of LPAR1 signaling, competing with GRK2 for LPAR1 binding, thus influencing receptor signaling. RALA is required for anchorage-independent proliferation of transformed cells. During mitosis, it supports intracellular bridge stabilization and elongation between dividing cells. It cooperates with EXOC2 to recruit other exocyst components to the early midbody. RALA also controls mitochondrial fission during mitosis by recruiting RALBP1 to the mitochondrion, which mediates DNM1L phosphorylation and activation by cyclin B-CDK1.

Protein Families

Small GTPase superfamily, Ras family

Subcellular Location

Cell membrane; Lipid-anchor; Cytoplasmic side. Cleavage furrow. Midbody, Midbody ring. Mitochondrion.

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Recombinant Saguinus oedipus Ras-related protein Ral-A (RALA)

Description

Overview of Human RALA

Key Functional Domains:

Role in Cellular Processes

Effector Interactions and Outcomes:

Cancer Associations:

Pharmacological Targeting:

Recombinant RALA Production (General Workflow)

Research Gaps and Recommendations

Product Specs

Target Background

Quick Inquiry

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Service