Recombinant Salmonella arizonae Urocanate hydratase (hutU), partial
Shipped with Ice Packs 
In Stock 
Description
Production and Purification
The enzyme is expressed in heterologous systems such as E. coli, yeast, or mammalian cells . Mammalian cell systems (e.g., HEK293) are preferred for proper post-translational modifications .
Key Production Parameters:
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Expression Vector: Customized for high-yield soluble protein .
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Purification: Affinity chromatography (e.g., Ni-NTA for His-tagged variants) .
Reconstitution Guidelines:
Research Applications
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Metabolic Pathway Analysis: Used to study histidine catabolism in Salmonella arizonae .
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Antimicrobial Target Screening: Explored for inhibiting bacterial growth in zoonotic infections .
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Enzyme Kinetics: Assayed for substrate specificity and inhibition kinetics .
Limitations and Future Directions
Product Specs
Form
Lyophilized powder. We will ship the available format, but please specify any format requirements when ordering.
Lead Time
Delivery times vary. Contact your local distributor for details. Proteins are shipped with blue ice packs. Request dry ice in advance (extra fees apply).
Notes
Avoid repeated freeze-thaw cycles. Working aliquots are stable at 4°C for up to one week.
Reconstitution
Briefly centrifuge the vial before opening. Reconstitute in sterile deionized water to 0.1-1.0 mg/mL. Add 5-50% glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. Default glycerol concentration is 50%.
Shelf Life
Shelf life depends on storage conditions, buffer, temperature, and protein stability. Liquid form: 6 months at -20°C/-80°C. Lyophilized form: 12 months at -20°C/-80°C.
Storage Condition
Store at -20°C/-80°C upon receipt. Aliquot for multiple uses. Avoid repeated freeze-thaw cycles.
Tag Info
Tag type is determined during manufacturing. Specify your preferred tag type, and we will prioritize its development.
Synonyms
hutU; SARI_02136; Urocanate hydratase; Urocanase; EC 4.2.1.49; Imidazolonepropionate hydrolase
Buffer Before Lyophilization
Tris/PBS-based buffer, 6%
Trehalose.
Datasheet
Please contact us to get it.
Protein Length
Partial
Purity
>85% (SDS-PAGE)
Species
Salmonella arizonae (strain ATCC BAA-731 / CDC346-86 / RSK2980)
Target Names
hutU
Uniprot No.
Target Background
Function
Converts urocanate to 4-imidazolone-5-propionate.
Database Links
KEGG: ses:SARI_02136
STRING: 882884.SARI_02136
Protein Families
Urocanase family
Subcellular Location
Cytoplasm.
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