Recombinant Salmonella choleraesuis Protein MgtC (mgtC)
Description
Introduction to Recombinant Salmonella choleraesuis Protein MgtC (mgtC)
Recombinant Salmonella choleraesuis Protein MgtC (mgtC) is a bacterial virulence factor expressed in E. coli for research purposes. This protein is critical for Salmonella’s survival within host macrophages and growth in magnesium-limited environments . The recombinant form is engineered with a His-tag to facilitate purification and functional studies .
2.2. Biological Roles
MgtC performs dual functions:
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ATP Regulation: Inhibits the F₁F₀ ATP synthase, reducing cytoplasmic ATP levels and stabilizing cytoplasmic pH .
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Pathogen Adaptation:
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pH Homeostasis: Maintains near-neutral pH in acidic macrophage environments .
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PhoP Stabilization: Prevents degradation of PhoP, a master regulator of virulence genes .
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Cellulose Repression: Lowers cyclic diguanylate (c-di-GMP), inhibiting cellulose biosynthesis to avoid biofilm formation during infection .
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Production and Purification
The recombinant protein is produced via heterologous expression in E. coli, leveraging codon optimization for high yield. Key steps include:
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Cloning: Insertion of the mgtC gene into a plasmid with a His-tag.
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Induction: IPTG-mediated expression under optimized conditions.
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Purification: Nickel-affinity chromatography to isolate His-tagged MgtC .
Regulatory Mechanisms
MgtC activity is tightly controlled by:
5.1. Virulence Studies
MgtC’s role in:
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Intracellular Survival: Required for replication within macrophages .
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Magnesium Depletion: Enables growth in low Mg²⁺ environments .
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Antibiotic Tolerance: Reduced ATP levels may enhance persistence under stress .
5.2. Functional Assays
6.1. Dual Roles in Host and Non-Host Environments
MgtC homologs from Yersinia pestis fully complement Salmonella ΔmgtC in macrophages, while Pseudomonas aeruginosa homologs only restore low Mg²⁺ growth . This highlights MgtC’s conserved and pathogen-specific functions.
6.2. mRNA Leader Regulation
The mgtCBR mRNA leader contains upstream ORFs (mgtM, mgtP) that modulate mgtC expression. Mutations in mgtM increase MgtC production, mimicking ΔLD (leader deletion) strains .
6.3. CigR Threshold Dynamics
CigR protein levels exceed MgtC early during infection, delaying virulence activation until MgtC accumulates . This threshold mechanism prevents premature ATP depletion in transient environments.
Product Specs
Please note that we will prioritize shipping the format currently in stock. However, if you have a specific format requirement, please indicate it in your order notes, and we will prepare the product accordingly.
All our proteins are shipped with standard blue ice packs. If you require dry ice shipping, please notify us in advance, as additional fees will apply.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Target Background
KEGG: sec:SCH_3685
