Recombinant Salmonella enteritidis PT4 UPF0761 membrane protein yihY (yihY)
Description
Production and Purification
The protein is produced in Escherichia coli via recombinant DNA technology . Key steps include:
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Cloning: The yihY gene is codon-optimized, synthesized, and subcloned into expression vectors .
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Expression: Induced using IPTG in E. coli BL21(DE3) or similar strains .
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Purification: Affinity chromatography (Ni-NTA columns) followed by buffer exchange and lyophilization .
Comparative Expression Systems
| Vendor | Host System | Purity | Tag |
|---|---|---|---|
| Creative BioMart | E. coli | >90% | His |
| MyBioSource | E. coli/Yeast/Mammalian | ≥85% | His or None |
| Gentaur | Custom (E. coli, mammalian) | >80% | His, GST, etc. |
Functional Role of Native YihY
YihY is a membrane-associated protein in Salmonella with potential roles in:
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Virulence: Membrane proteins like YihY contribute to bacterial survival in hostile environments (e.g., egg albumen) .
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Antimicrobial Resistance: Linked to genomic islands harboring resistance genes .
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Membrane Biogenesis: Homology to ribonuclease BN suggests involvement in RNA processing .
Vaccine Development
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Subunit vaccines: Recombinant YihY is tested as an antigen to elicit immune responses against Salmonella .
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Example: Immunoreactivity confirmed via western blot using antisera from Salmonella-infected hosts .
Diagnostic Tools
Key Research Findings
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Immunogenicity: Recombinant YihY reacts with antisera from Salmonella-infected hosts, confirming its antigenic potential .
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Genomic Context: The yihY gene is adjacent to virulence factors in pathogenicity islands (SPI-1 to SPI-5) .
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Structural Homology: Shares functional motifs with E. coli YidC, a membrane protein insertase .
Limitations and Future Directions
Product Specs
Note: While we prioritize shipping the format currently in stock, please specify your preferred format in order notes for customized preparation.
Note: Standard shipping includes blue ice packs. Dry ice shipping requires prior arrangement and incurs additional charges.
The tag type will be determined during the production process. To ensure a specific tag type, please inform us during your order; we will prioritize your request.
Target Background
KEGG: set:SEN3815
