Recombinant Salmonella heidelberg Glycine cleavage system H protein (gcvH)

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In Stock
Cat. No.
BT38386
Source
Yeast
Synonyms
gcvH; SeHA_C3286Glycine cleavage system H protein
Purity
>85% (SDS-PAGE)
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Cat. No.
BT38386
Source
E.coli
Synonyms
gcvH; SeHA_C3286Glycine cleavage system H protein
Purity
>85% (SDS-PAGE)
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Cat. No.
BT38386
Source
E.coli
Synonyms
gcvH; SeHA_C3286Glycine cleavage system H protein
Purity
>85% (SDS-PAGE)
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Cat. No.
BT38386
Source
Baculovirus
Synonyms
gcvH; SeHA_C3286Glycine cleavage system H protein
Purity
>85% (SDS-PAGE)
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Cat. No.
BT38386
Source
Mammalian cell
Synonyms
gcvH; SeHA_C3286Glycine cleavage system H protein
Purity
>85% (SDS-PAGE)
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Description

Recombinant S. heidelberg GcvH: Current Knowledge and Gaps

Recombinant GcvH production is typically achieved via heterologous expression in E. coli or yeast systems, followed by purification for biochemical or functional studies. While S. heidelberg GcvH has not been extensively characterized, its homology to E. coli and B. subtilis GcvH suggests conserved metabolic roles.

Key Properties of Recombinant GcvH

  • Molecular Weight: ~13–14 kDa (based on E. coli homolog )

  • Lipoylation: Critical for stability and function; lipoyl groups are transferred via the LipB pathway or GCS-mediated relay .

  • Subcellular Localization: Cytoplasmic, as part of the GCS complex .

Hypothesized Roles in S. heidelberg

  1. Metabolic Adaptation:

    • Facilitates glycine utilization under nutrient-limited conditions, supporting survival in host niches.

    • Integrates with folate biosynthesis pathways to sustain DNA synthesis during rapid growth.

  2. Pathogenicity:

    • Potential moonlighting functions analogous to Mycoplasma GcvH, such as modulating host cell stress responses (e.g., apoptosis inhibition).

    • Interaction with host proteins (e.g., kinases or chaperones) may enhance colonization or persistence.

Research Gaps and Future Directions

AreaCurrent StatusResearch Needs
Structural BiologyLimited crystallographic data for S. heidelberg GcvH; homology models available.X-ray crystallography or cryo-EM to resolve lipoylation-dependent conformations.
Functional AnnotationNo direct studies on S. heidelberg GcvH; inferred from E. coli and B. subtilis.Enzymatic assays (e.g., glycine decarboxylase activity) in S. heidelberg mutants.
PathogenicityNo evidence linking GcvH to virulence in S. heidelberg; speculative based on Mycoplasma.Host cell infection assays to test GcvH-driven apoptosis modulation.

Proposed Experimental Approaches

  • Functional Characterization:

    • Assess lipoylation efficiency using mass spectrometry.

    • Measure glycine cleavage activity in tandem with P- and T-proteins .

Therapeutic and Diagnostic Potential

While not yet validated, GcvH could serve as:

  • Vaccine Target: A conserved antigen for cross-strain protection.

  • Diagnostic Marker: Elevated GcvH expression in antibiotic-resistant strains may correlate with metabolic adaptation.

  • Drug Target: Inhibitors of GcvH-lipoyl relay could disrupt folate synthesis, limiting S. heidelberg proliferation.

Product Specs

Form
Lyophilized powder. We will ship the format we have in stock. If you have special format requirements, please note them when ordering.
Lead Time
Delivery time varies by purchasing method and location. Consult your local distributor for specific delivery times. All proteins are shipped with blue ice packs by default. For dry ice shipping, contact us in advance; extra fees apply.
Notes
Avoid repeated freeze-thaw cycles. Store working aliquots at 4°C for up to one week.
Reconstitution
Briefly centrifuge the vial before opening. Reconstitute in sterile deionized water to 0.1-1.0 mg/mL. Add 5-50% glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. Our default final glycerol concentration is 50%.
Shelf Life
Shelf life depends on storage conditions, buffer ingredients, storage temperature, and protein stability. Liquid form: 6 months at -20°C/-80°C. Lyophilized form: 12 months at -20°C/-80°C.
Storage Condition
Store at -20°C/-80°C upon receipt. Aliquot for multiple uses. Avoid repeated freeze-thaw cycles.
Tag Info
Tag type is determined during manufacturing. If you have a specific tag type requirement, please inform us, and we will prioritize developing it.
Synonyms
gcvH; SeHA_C3286Glycine cleavage system H protein
Buffer Before Lyophilization
Tris/PBS-based buffer, 6% Trehalose.
Datasheet
Please contact us to get it.
Expression Region
1-129
Protein Length
full length protein
Purity
>85% (SDS-PAGE)
Species
Salmonella heidelberg (strain SL476)
Target Names
gcvH
Target Protein Sequence
MSNVPAELKY SKEHEWLRKE ADGTYTVGIT EHAQELLGDM VFVDLPEVGA TVSAGDDCAV AESVKAASDI YAPVSGEIVA VNDALSDSPE LVNSEPYAGG WIFKIKASDE SELESLLDAT AYEALLEDE
Uniprot No.
B4TGX4

Target Background

Function
The glycine cleavage system degrades glycine. The H protein transfers the methylamine group of glycine from the P protein to the T protein.
Database Links

KEGG: seh:SeHA_C3286

Protein Families
GcvH family

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