Recombinant Salmonella typhimurium Secretion system apparatus protein SsaS (ssaS)
Description
Expression Systems
ssaS is produced via heterologous expression:
| Host Organism | Expression Vector | Purification Method | Source |
|---|---|---|---|
| E. coli | pET28a or similar | Ni-NTA affinity columns | |
| Yeast | Undisclosed | Undisclosed |
Role in Bacterial Pathogenicity
While ssaS-specific studies are sparse, secretion systems in Salmonella are pivotal for:
-
Type III Secretion System (T3SS): Invades host cells via needle-like structures (e.g., PrgI) .
-
Type I Secretion System (T1SS): Excretes large proteins like SiiE into host cells .
ssaS may contribute to structural or regulatory aspects of these systems, though functional data remain unreported.
Diagnostic and Therapeutic Potential
Recombinant ssaS could serve as:
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Antigen for Immune Studies: Similar to OmpA/D, which elicits CD8+ T-cell responses in reactive arthritis .
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Tool for Secretion Mechanism Studies: Analogous to SsaS homologs in other pathogens.
Table 2: Comparative Secretion System Components in Salmonella
| Protein | System | Function | Reference |
|---|---|---|---|
| SiiE | T1SS | Giant non-fimbrial adhesin | |
| PrgI | T3SS | Needle filament component | |
| SsaS | Undetermined | Secretion apparatus role |
Research Gaps and Future Directions
Current limitations include:
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Functional Ambiguity: No studies explicitly link ssaS to secretion pathway regulation or effector protein translocation.
-
Structural Data: No crystallographic or cryo-EM data available.
Future research could explore:
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Interaction Partners: Co-purification with T3SS/T1SS components.
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Immunogenicity: Potential as a vaccine target or diagnostic marker.
Product Specs
Note: While we prioritize shipping the format currently in stock, we are happy to accommodate specific format requests. Please indicate your desired format in the order notes and we will fulfill your requirement.
Note: All proteins are shipped with standard blue ice packs. If you require dry ice shipping, please communicate this in advance, as additional fees will apply.
Generally, liquid form has a shelf life of 6 months at -20°C/-80°C. Lyophilized form has a shelf life of 12 months at -20°C/-80°C.
Target Background
KEGG: stm:STM1420
STRING: 99287.STM1420
