Recombinant Schizosaccharomyces pombe Meiotically up-regulated gene 116 protein (mug116)
Description
Introduction to Recombinant Schizosaccharomyces pombe Meiotically Up-Regulated Gene 116 Protein (mug116)
The Recombinant Schizosaccharomyces pombe Meiotically Up-Regulated Gene 116 Protein (mug116) is a protein expressed in the fission yeast Schizosaccharomyces pombe. This protein is particularly noted for its role during meiosis, a critical phase of sexual reproduction in eukaryotic organisms where genetic material is halved to produce gametes . The recombinant form of this protein is produced in Escherichia coli (E. coli) for research purposes, often with a His-tag for easier purification .
Characteristics of Recombinant mug116 Protein
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Expression Host: The recombinant mug116 protein is expressed in E. coli, which is a common host for recombinant protein production due to its well-understood genetics and rapid growth rate .
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Protein Length: The full-length mug116 protein consists of 135 amino acids .
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Purity and Storage: The protein is available in a lyophilized form with a purity of greater than 90% as determined by SDS-PAGE. It is recommended to store the protein at -20°C or -80°C to maintain its integrity .
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Reconstitution: For use, the protein should be reconstituted in deionized sterile water to a concentration of 0.1-1.0 mg/mL, with the option to add glycerol for long-term storage .
Research Findings and Applications
| Characteristics | Description |
|---|---|
| Species | Schizosaccharomyces pombe |
| Expression Host | Escherichia coli |
| Protein Length | 135 amino acids |
| Purity | >90% (SDS-PAGE) |
| Storage | -20°C or -80°C |
| Role | Meiosis |
Future Directions
Future research should aim to elucidate the precise mechanisms by which mug116 influences meiotic processes. This could involve genetic studies to identify interacting partners or biochemical assays to determine its enzymatic activities. Additionally, exploring the conservation of mug116-like proteins across different species could provide broader insights into meiotic regulation.
Product Specs
Note: While we prioritize shipping the format currently in stock, please specify your preferred format in order notes for customized preparation.
Note: Standard shipping includes blue ice packs. Dry ice shipping requires prior arrangement and incurs additional charges.
The tag type is determined during production. If you require a specific tag, please inform us, and we will prioritize its development.
Target Background
KEGG: spo:SPAC5D6.10c
