Recombinant Schizosaccharomyces pombe Mitochondrial respiratory chain complexes assembly protein rca1 (SPBC543.09)
Description
Introduction to Recombinant Schizosaccharomyces pombe Mitochondrial Respiratory Chain Complexes Assembly Protein rca1 (SPBC543.09)
The recombinant Schizosaccharomyces pombe mitochondrial respiratory chain complexes assembly protein rca1 (SPBC543.09) is a crucial component involved in the assembly of mitochondrial respiratory chain complexes. These complexes are essential for the generation of ATP in eukaryotic cells, including those of Schizosaccharomyces pombe, a model organism often used in molecular biology research.
Function and Importance
The rca1 protein plays a significant role in ensuring the proper assembly and function of mitochondrial respiratory chain complexes. Mitochondrial respiratory chain complexes are vital for cellular respiration, where they facilitate the transfer of electrons and generate ATP through oxidative phosphorylation. In S. pombe, as in other eukaryotes, these complexes are crucial for energy metabolism and maintaining cellular homeostasis.
Protein Characteristics
The rca1 protein is encoded by the SPBC543.09 ORF in S. pombe. It has a specific amino acid sequence that defines its structure and function. The protein sequence includes various motifs and domains that are crucial for its role in assembling mitochondrial respiratory chain complexes.
| Protein Feature | Description |
|---|---|
| ORF Name | SPBC543.09 |
| Protein Name | rca1 |
| Function | Assembly of mitochondrial respiratory chain complexes |
| Sequence Length | 773 amino acids |
Experimental Tools and Techniques
For studying rca1 (SPBC543.09), researchers often use recombinant protein expression systems. These systems allow for the production of large quantities of the protein, which can then be purified and analyzed using techniques like ELISA (Enzyme-Linked Immunosorbent Assay) . S. pombe itself is a popular host for eukaryotic protein expression due to its ability to perform post-translational modifications, which are critical for protein function .
Product Specs
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Target Background
Recombinant Schizosaccharomyces pombe Mitochondrial respiratory chain complexes assembly protein rca1 (SPBC543.09) is a component of the m-AAA protease complex. This ATP-dependent metalloprotease degrades unassembled mitochondrial inner membrane proteins. The complex is essential for the assembly of mitochondrial respiratory chain and ATPase complexes. It functions in both post-translational assembly and the turnover of misfolded or mistranslated polypeptides.
KEGG: spo:SPBC543.09
STRING: 4896.SPBC543.09.1
