Recombinant Schizosaccharomyces pombe Mitochondrial tRNA-specific 2-thiouridylase 1 (SPAC23H4.04)

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Cat. No.
BT41657
Source
Yeast
Synonyms
SPAC23H4.04; Mitochondrial tRNA-specific 2-thiouridylase 1; EC 2.8.1.14
Purity
>85% (SDS-PAGE)
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Cat. No.
BT41657
Source
E.coli
Synonyms
SPAC23H4.04; Mitochondrial tRNA-specific 2-thiouridylase 1; EC 2.8.1.14
Purity
>85% (SDS-PAGE)
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Cat. No.
BT41657
Source
E.coli
Synonyms
SPAC23H4.04; Mitochondrial tRNA-specific 2-thiouridylase 1; EC 2.8.1.14
Purity
>85% (SDS-PAGE)
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Cat. No.
BT41657
Source
Baculovirus
Synonyms
SPAC23H4.04; Mitochondrial tRNA-specific 2-thiouridylase 1; EC 2.8.1.14
Purity
>85% (SDS-PAGE)
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Cat. No.
BT41657
Source
Mammalian cell
Synonyms
SPAC23H4.04; Mitochondrial tRNA-specific 2-thiouridylase 1; EC 2.8.1.14
Purity
>85% (SDS-PAGE)
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Description

Mitochondrial tRNA Modification in Yeast

Mitochondrial tRNAs undergo posttranscriptional modifications critical for accurate translation of mitochondrial DNA-encoded proteins. In S. pombe, enzymes like MTU1 (homologous to human TRMU) catalyze 2-thiolation of uridine at the wobble position (U34) of mt-tRNAs (Lys, Glu, Gln). This modification enhances codon-anticodon pairing fidelity . While human and bacterial homologs are well-studied, S. pombe-specific data remain limited.

Gene Nomenclature and Functional Ambiguity

The gene designation SPAC23H4.04 is not explicitly mentioned in indexed literature. Close examination of S. pombe gene databases reveals:

  • SPAC23H4.05c: An uncharacterized protein with no annotated function .

  • SPAC23H4.02/ppk9+: Encodes a Snf1-like protein kinase (Ssp2) involved in glucose derepression, unrelated to tRNA modification .

This discrepancy suggests either a misannotation, a novel gene, or a focus on an understudied locus.

Recombinant Protein Production and Challenges

Recombinant mitochondrial tRNA-modifying enzymes are typically produced in E. coli or yeast systems. For instance:

FeatureHuman TRMU Hypothetical SPAC23H4.04
Host SystemE. coliNot reported
TagC-terminal His-tagUnknown
Purity>85% (SDS-PAGE)Unverified
Activity2-thiolation of mt-tRNAsPresumed, unconfirmed

No commercial or academic sources (e.g., Creative BioMart , MyBioSource ) list SPAC23H4.04 as a recombinant product.

Research Gaps and Future Directions

  1. Functional Characterization:

    • No studies confirm SPAC23H4.04’s role in mitochondrial tRNA modification.

    • Phylogenetic comparison with human TRMU or bacterial MnmA could clarify homology.

  2. Enzymatic Validation:

    • In vitro assays (e.g., mt-tRNA 2-thiolation activity) are required to establish function.

  3. Disease Relevance:

    • Pathological mutations in human TRMU cause mitochondrial disorders . Similar studies in S. pombe could model disease mechanisms.

Product Specs

Form
Lyophilized powder. We will ship the in-stock format preferentially. If you have special format requirements, please note them when ordering.
Lead Time
Delivery time varies by purchase method and location. Consult local distributors for specific times. Proteins are shipped with blue ice packs by default. Request dry ice in advance (extra fees apply).
Notes
Avoid repeated freeze-thaw cycles. Store working aliquots at 4°C for up to one week.
Reconstitution
Briefly centrifuge the vial before opening. Reconstitute in sterile deionized water to 0.1-1.0 mg/mL. Add 5-50% glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. Our default final glycerol concentration is 50%.
Shelf Life
Shelf life depends on storage conditions, buffer, temperature, and protein stability. Liquid form: 6 months at -20°C/-80°C. Lyophilized form: 12 months at -20°C/-80°C.
Storage Condition
Store at -20°C/-80°C upon receipt. Aliquot for multiple uses. Avoid repeated freeze-thaw cycles.
Tag Info
Tag type is determined during manufacturing. If you require a specific tag, please inform us, and we will prioritize its development.
Synonyms
SPAC23H4.04; Mitochondrial tRNA-specific 2-thiouridylase 1; EC 2.8.1.14
Buffer Before Lyophilization
Tris/PBS-based buffer, 6% Trehalose.
Datasheet
Please contact us to get it.
Expression Region
1-415
Protein Length
full length protein
Purity
>85% (SDS-PAGE)
Species
Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast)
Target Names
SPAC23H4.04
Target Protein Sequence
MRVSLFLQKQ IIECSKAFQP HSTRLQWPKS QDKVFVAMSG GVDSSFSAYL LKSQGYNVEG VFMRNWLDED SAPSGCPAER DWATVQKVCK KLNISCRRFN FEKEYWNLVF EPSLDLYENG LTPNPDVSCN RQVKFGALFD ALKKHCENNV KGDWWLASGH YAKSVVNIET NESHMCIPTD KRKDQTLFLC TIRKEALEKT IFPLHNWTKE NVKKQASSAG FKEIAEKQES QGLCFVSPNV GRKFRKFLQR YLNFSDRPIK VIAGKNVVGE FSGNHGIWSL TVGERCGLSL PQAQSEYFGR WYVWKKDIKN NALYICRGTN NELLMSKCIY LKDWKWCGTK LQNLEKSALS CFVRVRHQQP LQPAKVTWRN PESVKIHFQD KQRAVTPGQV IAVYVNDVCL GGGMVDTVEP EKDFD
Uniprot No.
O13947

Target Background

Function
Catalyzes the 2-thiolation of uridine at position U34 of mitochondrial tRNA(Lys), tRNA(Glu), and tRNA(Gln). Required for the formation of 5-taurinomethyl-2-thiouridine (tm5s2U) at the wobble position of these tRNAs. ATP is needed to activate the C2 atom of the wobble base.
Database Links

KEGG: spo:SPAC23H4.04

STRING: 4896.SPAC23H4.04.1

Protein Families
MnmA/TRMU family
Subcellular Location
Mitochondrion.

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