Recombinant Schizosaccharomyces pombe Nuclear envelope morphology protein 1 (nem1)
Description
Introduction to Recombinant Schizosaccharomyces pombe Nuclear Envelope Morphology Protein 1 (nem1)
Recombinant Schizosaccharomyces pombe Nuclear Envelope Morphology Protein 1 (nem1) is a protein derived from the fission yeast Schizosaccharomyces pombe. This protein plays a crucial role in maintaining the structure and integrity of the nuclear envelope, which is essential for cellular processes such as mitosis and meiosis. The recombinant form of nem1 is produced through genetic engineering techniques, typically expressed in Escherichia coli (E. coli), and is used in various research applications to study nuclear envelope dynamics and cellular biology.
Characteristics of Recombinant nem1
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Protein Length and Structure: The recombinant nem1 protein is a full-length protein consisting of 476 amino acids. It is often fused with a His-tag at the N-terminal for easy purification and detection .
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Expression System: The protein is expressed in E. coli, which provides a cost-effective and efficient system for large-scale production .
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Purity and Storage: The recombinant nem1 is purified to a high degree, typically greater than 90% as determined by SDS-PAGE. It is stored as a lyophilized powder at -20°C or -80°C to maintain stability .
Biological Function of nem1
In Schizosaccharomyces pombe, the nuclear envelope remains intact throughout the cell cycle, a process known as "closed" mitosis . The nem1 protein is involved in maintaining this integrity by regulating nuclear envelope morphology. Although specific mechanisms are not fully elucidated, proteins like nem1 are crucial for ensuring proper nuclear compartmentalization and function during cell division.
Research Applications
Recombinant nem1 is used in research to study nuclear envelope dynamics, particularly in the context of Schizosaccharomyces pombe. This includes investigations into how the nuclear envelope maintains its structure during mitosis and meiosis, and how proteins like nem1 contribute to these processes.
Product Specs
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The tag type is finalized during production. If a specific tag is required, please inform us for preferential development.
Target Background
KEGG: spo:SPBC3B8.10c
STRING: 4896.SPBC3B8.10c.1
