Recombinant Schizosaccharomyces pombe Protein transport protein yip1 (yip1)
Description
Definition and Biological Context
Yip1 is an evolutionarily conserved integral membrane protein involved in ER-to-Golgi trafficking. In Schizosaccharomyces pombe (fission yeast), it interacts with transport GTPases (e.g., Ypt1p, Ypt31p) and forms a complex with Yif1p, a related transmembrane protein . This complex is essential for vesicle docking and fusion, as demonstrated in Saccharomyces cerevisiae, where loss of Yip1 function disrupts secretory pathways and causes ER membrane proliferation .
GTPase Interaction and Vesicle Transport
Yip1 binds GDP-bound Ypt GTPases (Ypt1p, Ypt31p) via its cytosolic domain, facilitating vesicle docking . In S. cerevisiae, conditional yip1 mutants accumulate ER membranes and 40–50 nm vesicles, indicating a block in ER-to-Golgi transport .
Genetic Interactions
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Synthetic lethality occurs between yip1 and yif1 mutants, confirming their functional interdependence .
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Overexpression of Yif1p suppresses yip1 mutant defects, suggesting Yip1 acts upstream of Yif1p in the transport pathway .
Morphological Defects
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Temperature-sensitive yip1 mutants exhibit ER membrane proliferation and secretory defects analogous to sec18 mutants (ER-to-Golgi block) .
Applications in Research
Product Specs
Note: We prioritize shipping the format currently in stock. However, if you have specific format requirements, please indicate them during order placement, and we will fulfill your request.
Note: All proteins are shipped with standard blue ice packs. If dry ice shipping is required, please inform us beforehand, as additional fees will apply.
Generally, the shelf life of the liquid form is 6 months at -20°C/-80°C. The shelf life of the lyophilized form is 12 months at -20°C/-80°C.
Target Background
KEGG: spo:SPCC61.04c
STRING: 4896.SPCC61.04c.1
