Recombinant Schizosaccharomyces pombe Protein YIP4 (SPAC644.13c)
Description
Genomic Context and Expression
SPAC644.13c is annotated in the S. pombe genome, though its functional role remains uncharacterized in the reviewed literature. Key observations include:
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Expression Profiling: SPAC644.13c is not highlighted in mRNA expression datasets from vegetatively growing cells , suggesting low expression under standard conditions.
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Chromatin Interactions: Proteins encoded by neighboring loci (e.g., transcription factors like Atf1, Rst2) are enriched in highly occupied target (HOT) regions , which may imply indirect regulatory roles for adjacent genes.
Protein Interaction Networks
While YIP4 itself is not discussed, systematic interaction studies in S. pombe provide frameworks for hypothesizing its function:
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TF-Protein Interactions: Approximately 50% of S. pombe transcription factors (TFs) exhibit physical interactions with co-activators, chromatin remodelers, or metabolic enzymes . Proteins lacking DNA-binding domains (like YIP4) often partner with TFs to modulate activity.
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14-3-3 Protein Interactions: Many TFs and regulatory proteins bind 14-3-3 phospho-binding proteins , a conserved mechanism for post-translational regulation.
Comparative Proteomics and Secretion
Studies on recombinant protein production in S. pombe highlight challenges and strategies relevant to expressing proteins like YIP4:
Table 1: Key Factors Affecting Recombinant Protein Secretion in S. pombe
Technical Insights for Recombinant YIP4 Production
Based on methodologies in the reviewed studies:
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Strain Engineering: Endogenous tagging (e.g., epitope tags) enables precise immunoprecipitation and mass spectrometry (IP-MS) analysis .
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Chromatin Profiling: ChIP-seq could map YIP4’s genomic targets if it associates with DNA-binding partners .
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Proteogenomics: Integrating MS/MS data with six-frame genome translations identifies novel peptides and corrects gene models , a strategy applicable to characterizing YIP4.
Unresolved Questions and Future Directions
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Functional Annotation: SPAC644.13c lacks Pfam domains or homology to characterized proteins, necessitating de novo functional studies.
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Interaction Partners: IP-MS screens using tagged YIP4 could identify binding partners (e.g., TFs, metabolic enzymes) .
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Subcellular Localization: Fluorescent tagging may clarify whether YIP4 localizes to membranes, nuclei, or stress granules.
Product Specs
Note: While we will prioritize shipping the format currently in stock, we understand that you may have specific requirements. If so, please indicate your preference when placing the order, and we will prepare your product accordingly.
Note: All our proteins are shipped with standard blue ice packs. If you require dry ice shipping, please inform us in advance as additional fees will apply.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Target Background
KEGG: spo:SPAC644.13c
STRING: 4896.SPAC644.13c.1
