Recombinant Shewanella pealeana Argininosuccinate synthase (argG)
Description
Product Overview
Recombinant Shewanella pealeana ArgG (UniProt ID: A8GZ21) is a purified protein produced via bacterial expression systems. Key specifications include:
Catalytic Mechanism
ArgG facilitates the ATP-dependent ligation of citrulline and aspartate, forming argininosuccinate—a key step in arginine biosynthesis and nitrogen metabolism. Comparative studies with human argininosuccinate synthase reveal conserved active-site residues, though bacterial isoforms exhibit distinct regulatory mechanisms .
Biotechnological Applications
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Enzyme Engineering: Serves as a model for studying microbial urea-cycle adaptations .
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Metabolic Studies: Used to probe arginine auxotrophy in genetically modified organisms .
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Structural Biology: Crystal structures of homologs (e.g., E. coli ArgG) inform drug design targeting bacterial metabolism .
Genomic Context in Shewanella
While S. pealeana ArgG itself is not directly linked to antimicrobial resistance (AMR), genomic analyses of related Shewanella species highlight their propensity for horizontal gene transfer (HGT), including plasmids and integrons carrying AMR genes . This underscores the importance of studying metabolic enzymes like ArgG in environmentally resilient bacteria .
Bacterial vs. Human ArgG
Future Directions
Further studies could explore:
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Structural Dynamics: Cryo-EM or X-ray crystallography of S. pealeana ArgG to resolve substrate-binding mechanisms.
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Metabolic Flux Analysis: Role in nitrogen cycling under anaerobic conditions, relevant to bioremediation .
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Synthetic Biology: Integration into arginine-overproducing microbial chassis for industrial applications .
Product Specs
Target Background
KEGG: spl:Spea_0230
STRING: 398579.Spea_0230
