Recombinant Simulium vittatum NADH-ubiquinone oxidoreductase chain 4 (ND4)
Description
Molecular Structure and Function
Core Characteristics
1.2 Functional Role
ND4 is integral to Complex I, catalyzing the oxidation of NADH to NAD⁺ and transferring electrons to ubiquinone. This reaction contributes to proton translocation across the mitochondrial membrane, driving ATP synthesis .
Production and Purification
2.1 Expression System
The recombinant ND4 is expressed in E. coli using optimized vectors to ensure high yield and proper folding. Key production parameters include:
| Parameter | Detail | Source |
|---|---|---|
| Host Organism | E. coli | |
| Purity | >90% (SDS-PAGE validated) | |
| Form | Lyophilized powder | |
| Storage Buffer | Tris/PBS-based buffer with 6% trehalose, pH 8.0 |
Post-Production Handling
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Reconstitution: Dissolve in deionized sterile water (0.1–1.0 mg/mL) with 5–50% glycerol for stability .
Research Applications
Experimental Uses
| Application | Method/Outcome | Source |
|---|---|---|
| SDS-PAGE Analysis | Purity validation (>90% band intensity) | |
| ELISA Development | Antigen for antibody production | |
| Functional Assays | Studying electron transport chain dynamics |
3.2 Comparative Analysis
The Simulium vittatum ND4 shares structural homology with human ND4 (UniProt ID: P03905), though differences in sequence and membrane topology exist .
Key Research Findings
Stability and Handling
Product Specs
Note: While we prioritize shipping the format currently in stock, please specify your preferred format in order notes for customized preparation.
Note: All proteins are shipped with standard blue ice packs. Dry ice shipping requires advance notice and incurs additional charges.
The tag type is determined during production. If you require a specific tag, please inform us, and we will prioritize its inclusion.
Target Background
Core subunit of the mitochondrial membrane respiratory chain NADH dehydrogenase (Complex I). It's considered part of the minimal assembly required for catalytic activity. Complex I facilitates electron transfer from NADH to the respiratory chain, with ubiquinone believed to be the immediate electron acceptor.
