Recombinant Solanum lycopersicum 80 kDa cell wall protein

Shipped with Ice Packs
In Stock
Cat. No.
BT44696
Source
Yeast
Synonyms
80 kDa cell wall protein; Fragment
Purity
>85% (SDS-PAGE)
Quick Inquiry
Cat. No.
BT44696
Source
E.coli
Synonyms
80 kDa cell wall protein; Fragment
Purity
>85% (SDS-PAGE)
Quick Inquiry
Cat. No.
BT44696
Source
E.coli
Synonyms
80 kDa cell wall protein; Fragment
Purity
>85% (SDS-PAGE)
Quick Inquiry
Cat. No.
BT44696
Source
Baculovirus
Synonyms
80 kDa cell wall protein; Fragment
Purity
>85% (SDS-PAGE)
Quick Inquiry
Cat. No.
BT44696
Source
Mammalian cell
Synonyms
80 kDa cell wall protein; Fragment
Purity
>85% (SDS-PAGE)
Quick Inquiry

Description

Observed Molecular Weight Discrepancies

No proteins matching the 80 kDa specification were identified in Solanum lycopersicum. Documented recombinant cell wall-associated proteins include:

Protein NameMolecular WeightFunctionSource
Asc-1 (ASC1)~34 kDaAlternaria stem canker resistance
21 kDa cell wall protein21 kDaStructural cell wall component
SlHBP223 kDaHeme-binding, antimicrobial

Recombinant 21 kDa Cell Wall Protein

  • Expression System: Produced in E. coli, yeast, or mammalian cells .

  • Purity: ≥85% (SDS-PAGE verified) .

  • Storage: Lyophilized or liquid, stable at -20°C/-80°C .

  • Sequence: Uniprot ID P80803 (76364141) .

Recombinant SlHBP2 (23 kDa)

  • Antimicrobial Activity: Inhibits Pseudomonas syringae at 3–25 μg/mL, disrupts bacterial cell walls .

  • Heme Binding: Confirmed via hemin-agarose assays; critical tryptophan residues (W57, W211) mediate binding .

Challenges in Recombinant Cell Wall Protein Production

  • Solubility: Glycosyltransferases (GTs) often exhibit <5% soluble expression in E. coli .

  • Optimization Strategies:

    • Co-expression with chaperones (e.g., GroEL/ES) .

    • Buffer additives (e.g., glycerol, trehalose) to enhance stability .

    • Codon optimization and truncation of transmembrane domains .

Recommendations for Further Investigation

  1. Gene Database Mining: Query NCBI/Uniprot for hypothetical S. lycopersicum proteins near 80 kDa.

  2. Ortholog Analysis: Compare with known 70–90 kDa cell wall proteins in Arabidopsis (e.g., expansins, extensins).

  3. Experimental Validation:

    • Design primers for candidate genes using SL4.0 genome assembly.

    • Optimize expression in E. coli BL21(DE3) with pET vectors.

    • Validate via SDS-PAGE and Western blotting.

Product Specs

Form
Lyophilized powder. We will ship the available format, but if you have specific format requirements, please note them when ordering, and we will fulfill your request.
Lead Time
Delivery times vary by purchase method and location. Consult your local distributor for specific delivery times. All proteins are shipped with standard blue ice packs. For dry ice shipment, please contact us in advance, as additional charges apply.
Notes
Avoid repeated freezing and thawing. Working aliquots can be stored at 4°C for up to one week.
Reconstitution
Briefly centrifuge the vial before opening to collect the contents at the bottom. Reconstitute the protein in sterile deionized water to a concentration of 0.1-1.0 mg/mL. We recommend adding 5-50% glycerol (final concentration) and aliquoting for long-term storage at -20°C/-80°C. Our default final glycerol concentration is 50%.
Shelf Life
Shelf life depends on several factors, including storage conditions, buffer components, storage temperature, and protein stability. Generally, the liquid form has a shelf life of 6 months at -20°C/-80°C, while the lyophilized form has a shelf life of 12 months at -20°C/-80°C.
Storage Condition
Store at -20°C/-80°C upon receiving. Aliquot for multiple uses. Avoid repeated freeze-thaw cycles.
Tag Info
The tag type will be determined during the manufacturing process. If you have a specific tag type requirement, please inform us, and we will prioritize developing it.
Synonyms
80 kDa cell wall protein; Fragment
Buffer Before Lyophilization
Tris/PBS-based buffer, 6% Trehalose.
Datasheet
Please contact us to get it.
Expression Region
1-10
Protein Length
full length protein
Purity
>85% (SDS-PAGE)
Species
Solanum lycopersicum (Tomato) (Lycopersicon esculentum)
Target Protein Sequence
STHTSDFLKL
Uniprot No.
P80814

Target Background

Subcellular Location
Secreted, cell wall.

Quick Inquiry

Personal Email Detected
Please use an institutional or corporate email address for inquiries. Personal email accounts ( such as Gmail, Yahoo, and Outlook) are not accepted. *

Category

Service

THE BIOTEK
THE BioTek is a global biotech company offering highly purified proteins for research in cancer, apoptosis, development, endocrinology, immunology, neuroscience, proteases, and stem cells.
  • Contact
  • Address: 1925 E Maple Ave, El Segundo, CA 90245 United States
  • Phone : +1 201-285-7826
  • Email : info@thebiotek.com
  • Hours : Mon.-Fri. 9:00 AM - 5:00 PM
© Copyright 2025 TheBiotek. All Rights Reserved.