Recombinant Solanum lycopersicum NAD (P)H-quinone oxidoreductase subunit 6, chloroplastic (ndhG)
Description
Molecular Characterization
Gene ID: Q9MUL3 (UniProt)
Amino Acid Sequence: 189 residues (1-189aa) with N-terminal His tag for purification
Expression System: Escherichia coli
Domains:
| Property | Value | Source |
|---|---|---|
| Molecular Weight | ~22 kDa (predicted) | |
| Subcellular Localization | Chloroplast thylakoid membrane | |
| Structural Role | Core subunit of NDH complex |
Functional Role in Photosynthesis
The NDH complex facilitates two critical processes:
-
Cyclic Electron Transport (CET): Transfers electrons from NAD(P)H to plastoquinone, generating ATP without NADPH accumulation .
-
Chlororespiration: Maintains redox homeostasis under stress by balancing stromal NADPH/ATP ratios .
Key Biochemical Activities:
-
Electron Transfer: Mediates electron shuttling via FMN and iron-sulfur clusters .
-
Proton Translocation: Couples redox reactions to create proton gradients for ATP synthesis .
-
Stress Response: Upregulated under drought, high/low temperature, and oxidative stress to mitigate ROS .
Recombinant Production and Applications
Expression Protocol:
-
Codon-optimized ndhG cloned into pET vectors.
-
Induced with IPTG in E. coli, followed by Ni-NTA affinity chromatography .
Research Applications:
-
Mechanistic Studies: Elucidating NDH-PSI supercomplex assembly .
-
Stress Tolerance Engineering: Overexpression in transgenic tomatoes enhances resilience to abiotic stressors .
-
Biophysical Analyses: Structural resolution via cryo-EM to map electron transport pathways .
Expression Under Stress:
| Stress Condition | NDH Activity Change | Reference |
|---|---|---|
| High Light Intensity | ↑ 1.5–2.0-fold | |
| Drought | ↑ 1.8-fold | |
| Salt Stress | ↑ 1.49-fold |
Genetic Interactions:
-
Co-expressed with SlAREB1 (ABA-responsive TF) to enhance drought tolerance .
-
Silencing ndhG disrupts CET, increasing ROS accumulation under fluctuating light .
Challenges and Future Directions
-
Structural Complexity: The NDH complex comprises 35 subunits, complicating recombinant reconstitution .
-
Functional Redundancy: Overlaps with PGR5/PGRL1 pathways require conditional knockout studies .
-
Biotechnological Potential: Engineering NDH-CET efficiency could improve crop yields under climate stress .
Product Specs
Note: While we prioritize shipping the format currently in stock, please specify your format preference during order placement for fulfillment based on your requirements.
Note: All proteins are shipped with standard blue ice packs. Dry ice shipping requires prior arrangement and incurs additional charges.
The tag type is determined during production. Please specify your desired tag type for preferential development.
Target Background
KEGG: sly:3950402
STRING: 4081.Solyc03g013600.1.1
