Recombinant Solanum tuberosum Cytochrome b-c1 complex subunit 6

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Cat. No.
BT48104
Source
Yeast
Synonyms
; Cytochrome b-c1 complex subunit 6; CR7; Complex III subunit 6; Complex III subunit VI; Mitochondrial hinge protein; Ubiquinol-cytochrome c reductase complex 7.8 kDa protein
Purity
>85% (SDS-PAGE)
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Cat. No.
BT48104
Source
E.coli
Synonyms
; Cytochrome b-c1 complex subunit 6; CR7; Complex III subunit 6; Complex III subunit VI; Mitochondrial hinge protein; Ubiquinol-cytochrome c reductase complex 7.8 kDa protein
Purity
>85% (SDS-PAGE)
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Cat. No.
BT48104
Source
E.coli
Synonyms
; Cytochrome b-c1 complex subunit 6; CR7; Complex III subunit 6; Complex III subunit VI; Mitochondrial hinge protein; Ubiquinol-cytochrome c reductase complex 7.8 kDa protein
Purity
>85% (SDS-PAGE)
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Cat. No.
BT48104
Source
Baculovirus
Synonyms
; Cytochrome b-c1 complex subunit 6; CR7; Complex III subunit 6; Complex III subunit VI; Mitochondrial hinge protein; Ubiquinol-cytochrome c reductase complex 7.8 kDa protein
Purity
>85% (SDS-PAGE)
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Cat. No.
BT48104
Source
Mammalian cell
Synonyms
; Cytochrome b-c1 complex subunit 6; CR7; Complex III subunit 6; Complex III subunit VI; Mitochondrial hinge protein; Ubiquinol-cytochrome c reductase complex 7.8 kDa protein
Purity
>85% (SDS-PAGE)
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Description

Recombinant Production and Applications

Recombinant subunit 6 has been expressed using Agrobacterium tumefaciens-mediated transient expression in Nicotiana benthamiana and Escherichia coli systems . Key findings include:

  • Expression Systems:

    • E. coli: Produces soluble apoprotein requiring cofactor insertion in vitro .

    • Plant-based systems: Enable proper folding and post-translational modifications .

  • Functional Insights:

    • Overexpression in plants induces mitochondrial dysfunction and cell death, highlighting its regulatory role in redox signaling .

    • Acts as a pathogenicity target for fungal effectors (e.g., Sclerotinia sclerotiorum SsSSVP1), which disrupt its mitochondrial localization .

3.1. Pathogen Interaction Mechanisms

Subunit 6 is hijacked by fungal effectors to suppress host defenses:

  • SsSSVP1 Interaction: The S. sclerotiorum effector SsSSVP1 binds to subunit 6, redirecting it to the cytoplasm instead of mitochondria. This disrupts bc<sub>1</sub> complex assembly, leading to ROS accumulation and cell death .

  • Critical Cysteine Residues: Mutations at C<sup>38</sup> and C<sup>44</sup> in subunit 6 abolish its dimerization and effector binding, confirming the importance of redox-sensitive sites .

3.2. Role in Somatic Hybrids

Mitochondrial genome recombination in S. tuberosum somatic hybrids (StSc) influences subunit 6 gene conservation:

  • Gene Conservation: Subunit 6 genes are retained across parental (S. commersonii, S. tuberosum) and hybrid mitogenomes, indicating evolutionary stability .

  • NUMT Detection: 10.1–16.3% of hybrid mitochondrial sequences align with nuclear DNA, suggesting potential lateral gene transfer events affecting subunit 6 regulation .

Comparative Analysis Across Species

Subunit 6 homologs exhibit conserved roles but species-specific features:

OrganismHomologKey Differences
S. tuberosumQCR8Contains twin CX<sub>9</sub>C motifs; targeted by fungal effectors
Saccharomyces cerevisiaeQcr6Lacks cleavable presequence; required for Cyt1 proteolytic maturation
Rhodobacter sphaeroides-Absent; bc<sub>1</sub> complex lacks supernumerary subunits

Implications for Agricultural Biotechnology

  • Disease Resistance Engineering: Disrupting effector-subunit 6 interactions could mitigate fungal infections in crops .

  • Mitochondrial Engineering: Recombinant subunit 6 expression systems enable studies on bc<sub>1</sub> complex assembly in hybrids .

Product Specs

Form
Lyophilized powder. We will ship the format we have in stock. If you have special format requirements, please note them when ordering.
Lead Time
Delivery times vary by purchase method and location. Consult local distributors for specific delivery times. All proteins are shipped with blue ice packs by default. Contact us in advance for dry ice shipping (extra fees apply).
Notes
Avoid repeated freezing and thawing. Working aliquots are stable at 4°C for up to one week.
Reconstitution
Briefly centrifuge the vial before opening. Reconstitute in sterile deionized water to 0.1-1.0 mg/mL. Add 5-50% glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. Our default final glycerol concentration is 50%.
Shelf Life
Shelf life depends on storage conditions, buffer components, temperature, and protein stability. Liquid form: 6 months at -20°C/-80°C. Lyophilized form: 12 months at -20°C/-80°C.
Storage Condition
Store at -20°C/-80°C upon receipt. Aliquot for multiple uses. Avoid repeated freeze-thaw cycles.
Tag Info
Tag type is determined during manufacturing. If you require a specific tag, please inform us, and we will prioritize its development.
Synonyms
; Cytochrome b-c1 complex subunit 6; CR7; Complex III subunit 6; Complex III subunit VI; Mitochondrial hinge protein; Ubiquinol-cytochrome c reductase complex 7.8 kDa protein
Buffer Before Lyophilization
Tris/PBS-based buffer, 6% Trehalose.
Datasheet
Please contact us to get it.
Expression Region
2-69
Protein Length
Full Length of Mature Protein
Purity
>85% (SDS-PAGE)
Species
Solanum tuberosum (Potato)
Target Protein Sequence
SDEEVVDPK ATLEVSCKPK CVRQLKEYQA CTKRVEGDES GHKHCTGQYF DYWHCIDKCV AAKLFDHLK
Uniprot No.
P48504

Target Background

Function
This protein is part of the ubiquinol-cytochrome c oxidoreductase (cytochrome b-c1 complex, complex III), a component of the mitochondrial electron transport chain involved in oxidative phosphorylation. This chain, comprising complexes II, III, and IV, transfers electrons from NADH and succinate to oxygen, generating an electrochemical gradient that drives ATP synthesis. Complex III catalyzes electron transfer from ubiquinol to cytochrome c, coupling this with proton translocation across the inner membrane via the Q cycle. This process consumes 2 protons from the matrix, releases 4 protons into the intermembrane space, and transfers 2 electrons to cytochrome c.
Database Links

UniGene: Stu.18190

Protein Families
UQCRH/QCR6 family
Subcellular Location
Mitochondrion inner membrane; Peripheral membrane protein; Intermembrane side.

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