Recombinant Spinacia oleracea Chloroplast envelope membrane protein (cemA)
Description
Introduction
The Recombinant Spinacia oleracea Chloroplast Envelope Membrane Protein (cemA) is a key transmembrane protein localized to the inner envelope membrane of spinach chloroplasts. It plays a critical role in chloroplast physiology, particularly in ion transport and maintaining stromal pH during photosynthesis . Recombinant production enables detailed structural and functional studies, advancing research on chloroplast metabolism and bioengineering.
Protein Structure
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Sequence: 229 amino acids (1-229aa) , with a full-length sequence including transmembrane domains critical for membrane integration .
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Domains: Predicted multiple α-helical transmembrane regions, consistent with its role as an integral membrane transporter .
Physicochemical Properties
| Parameter | Value |
|---|---|
| Molecular Weight | ~27–28 kDa (theoretical) |
| Isoelectric Point (pI) | >8.8 (basic) |
| Expression System | E. coli (His-tagged) |
| Purity | >90% (SDS-PAGE verified) |
Expression and Purification
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Host: Expressed in E. coli with an N-terminal His tag for affinity chromatography .
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Form: Lyophilized powder in Tris/PBS-based buffer (pH 8.0) with 6% trehalose .
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Reconstitution: Solubilized in sterile water (0.1–1.0 mg/mL) with optional glycerol (5–50%) for stability .
Role in Chloroplast Physiology
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Proton Extrusion: Indirectly supports inorganic carbon uptake into chloroplasts, essential for photosynthesis .
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Transport Mechanisms: Part of a network of inner envelope transporters regulating metabolite flux and pH balance .
Enzymatic and Redox Activities
While cemA itself is not an enzyme, chloroplast envelope membranes host redox-active components (e.g., Fe-S centers and semiquinone radicals) . cemA’s structural role may facilitate interactions with these systems to regulate electron transfer and oxidative pathways .
Key Uses
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Antibody Production: Immunogen for raising antibodies against chloroplast envelope proteins .
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Structural Studies: Analysis of transmembrane topology and interactions .
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Functional Assays: Reconstitution in liposomes to study transport kinetics .
Comparative Analysis of Chloroplast Envelope Transporters
Future Directions
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Mechanistic Studies: Elucidate cemA’s role in carbon-concentrating mechanisms.
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Biotechnological Applications: Engineer chloroplast transporters to enhance photosynthetic efficiency in crops.
Product Specs
Please note: We will prioritize shipping the format currently in stock. However, if you have a specific format requirement, please indicate it in your order notes. We will strive to fulfill your request.
Note: All of our proteins are shipped with standard blue ice packs. If you require dry ice shipping, please communicate with us in advance as additional fees will apply.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Target Background
KEGG: soe:2715581
