Recombinant Staphylococcus aureus 50S ribosomal protein L7/L12 (rplL)

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Cat. No.

BT46527

Source

Yeast

Synonyms

rplL; SA0498; 50S ribosomal protein L7/L12

Purity

>85% (SDS-PAGE)

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Cat. No.

BT46527

Source

E.coli

Synonyms

rplL; SA0498; 50S ribosomal protein L7/L12

Purity

>85% (SDS-PAGE)

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Cat. No.

BT46527

Source

E.coli

Synonyms

rplL; SA0498; 50S ribosomal protein L7/L12

Purity

>85% (SDS-PAGE)

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Cat. No.

BT46527

Source

Baculovirus

Synonyms

rplL; SA0498; 50S ribosomal protein L7/L12

Purity

>85% (SDS-PAGE)

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Cat. No.

BT46527

Source

Mammalian cell

Synonyms

rplL; SA0498; 50S ribosomal protein L7/L12

Purity

>85% (SDS-PAGE)

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Description

Definition and Structure

Gene Origin: The rplL gene encodes a 122-amino-acid protein (MANHEQIIEAIKEMSVLELN...LK) with a molecular weight of 28.7 kDa .
Ribosomal Role: L7/L12 forms a pentameric structure (L7/L12)₅ in the ribosomal stalk, facilitating interactions with elongation factors (e.g., EF-G, EF-Tu) to regulate translation speed and accuracy .
Expression Hosts: Recombinant production is achieved in E. coli, yeast, baculovirus, or mammalian cells, with tags such as His-SUMO or AviTag for purification .

Expression and Purification

Parameter	Details	Source
Host Systems	E. coli, yeast, baculovirus, mammalian cells
Purity	≥85% (SDS-PAGE) for E. coli; ≥90% for yeast/mammalian systems
Storage	Lyophilized: -20°C/-80°C; liquid: -20°C/-80°C
Tag Information	N-terminal His-SUMO or C-terminal AviTag

Research Use

Antibiotic Resistance Studies: Mutations in rplL (e.g., Gly152Asp, Gly155Arg) are linked to resistance to oxazolidinones (linezolid, torezolid) and other antibiotics .
Diagnostic Development: Detecting L7/L12 in urine or lung homogenates serves as a biomarker for Streptococcus pneumoniae infections, with levels correlating to bacterial load .

Research Findings

Study Focus	Key Discoveries	Citations
Antibiotic Resistance	Mutations in rplL confer resistance to macrolides and oxazolidinones .
Diagnostic Utility	Urine-based assays detect L7/L12 in S. pneumoniae infections with high specificity .
Protein Abundance	L7/L12 levels increase proportionally with bacterial growth rate .

Product Specs

Form

Lyophilized powder. We will ship the format we have in stock. If you have special format requirements, please note them when ordering, and we will fulfill your request.

Lead Time

Delivery time varies by purchase method and location. Consult your local distributor for specific delivery times. All proteins are shipped with blue ice packs by default. For dry ice shipment, please contact us in advance; extra fees apply.

Notes

Avoid repeated freezing and thawing. Store working aliquots at 4°C for up to one week.

Reconstitution

Briefly centrifuge the vial before opening to collect contents at the bottom. Reconstitute the protein in sterile deionized water to 0.1-1.0 mg/mL. Add 5-50% glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. Our default final glycerol concentration is 50%.

Shelf Life

Shelf life depends on storage conditions, buffer components, storage temperature, and protein stability. Generally, the liquid form has a 6-month shelf life at -20°C/-80°C, while the lyophilized form has a 12-month shelf life at -20°C/-80°C.

Storage Condition

Store at -20°C/-80°C upon receipt. Aliquot for multiple uses. Avoid repeated freeze-thaw cycles.

Tag Info

The tag type will be determined during production. If you have a specific tag type requirement, please inform us, and we will prioritize developing it.

Synonyms

rplL; SA0498; 50S ribosomal protein L7/L12

Buffer Before Lyophilization

Tris/PBS-based buffer, 6% Trehalose.

Datasheet

Please contact us to get it.

Expression Region

1-122

Protein Length

full length protein

Purity

>85% (SDS-PAGE)

Species

Staphylococcus aureus (strain N315)

Target Names

rplL

Target Protein Sequence

MANHEQIIEA IKEMSVLELN DLVKAIEEEF GVTAAAPVAV AGAAGGADAA AEKTEFDVEL TSAGSSKIKV VKAVKEATGL GLKDAKELVD GAPKVIKEAL PKEEAEKLKE QLEEVGATVE LK

Uniprot No.

P99154

Target Background

Function

This protein is part of the ribosomal stalk, which facilitates interaction between the ribosome and GTP-bound translation factors. It is essential for accurate translation.

Database Links

KEGG: sau:SA0498

Protein Families

Bacterial ribosomal protein bL12 family

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Recombinant Staphylococcus aureus 50S ribosomal protein L7/L12 (rplL)

Description

Definition and Structure

Expression and Purification

Research Use

Research Findings

Product Specs

Target Background

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