Recombinant Staphylococcus aureus Probable manganese-dependent inorganic pyrophosphatase (ppaC)
Description
Introduction to Recombinant Staphylococcus aureus Probable Manganese-Dependent Inorganic Pyrophosphatase (ppaC)
Recombinant Staphylococcus aureus Probable Manganese-Dependent Inorganic Pyrophosphatase (ppaC) is an enzyme produced through recombinant DNA technology. This enzyme is crucial for the hydrolysis of inorganic pyrophosphate (PPi), a byproduct of various metabolic processes, into inorganic phosphate (Pi). The manganese dependency of this enzyme highlights its role in maintaining cellular homeostasis and metabolic balance within the bacterium.
Function and Importance of ppaC
The ppaC enzyme, encoded by the gene SAV1919 in Staphylococcus aureus, is a manganese-dependent inorganic pyrophosphatase. It plays a vital role in the bacterial metabolism by regulating the levels of inorganic pyrophosphate, which is essential for energy metabolism and nucleotide synthesis. The enzyme's activity is dependent on manganese ions (Mn²⁺), which act as cofactors to facilitate the hydrolysis reaction.
Research Findings
Recent studies have emphasized the significance of manganese-dependent enzymes in bacterial pathogenesis. For instance, manganese is crucial for the activity of certain enzymes involved in resisting oxidative stress and nutritional immunity in Staphylococcus aureus . Although specific research on ppaC is limited, its manganese dependency suggests it could be influenced by host-imposed manganese starvation, a defense mechanism known as nutritional immunity.
Table: Characteristics of Recombinant Staphylococcus aureus ppaC
| Characteristic | Description |
|---|---|
| Source | Produced in Yeast, E. coli, Baculovirus, or Mammalian cells |
| Function | Hydrolysis of inorganic pyrophosphate to inorganic phosphate |
| Cofactor | Manganese ions (Mn²⁺) |
| Gene | SAV1919 |
| KEGG Identifier | sav:SAV1919 |
| STRING Identifier | 158878.SAV1919 |
Production and Applications
Recombinant ppaC is commercially available and can be produced in various expression systems, including yeast, E. coli, baculovirus, and mammalian cells . This versatility allows for different applications in research and biotechnology, such as studying bacterial metabolism and developing diagnostic tools.
Product Specs
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Target Background
KEGG: sas:SAS1843
