Recombinant Staphylococcus aureus UPF0060 membrane protein SAHV_2323 (SAHV_2323)
Description
Understanding UPF0060 Proteins
UPF0060 proteins belong to a group of uncharacterized proteins that are conserved across different bacterial species. They are typically membrane-associated and may play roles in maintaining membrane integrity or facilitating interactions with other proteins or molecules. The specific function of SAHV_2323 would require detailed biochemical and structural studies.
Recombinant Expression
Recombinant expression of proteins like SAHV_2323 involves cloning the gene into an expression vector and expressing it in a host organism, often Escherichia coli. This process allows for the production of large quantities of the protein for further study. Recombinant proteins are frequently tagged with markers like His-tags to facilitate purification and identification.
Research Findings and Challenges
While specific research findings on SAHV_2323 are not readily available, studies on similar UPF0060 proteins suggest that they may be involved in membrane organization and stability. Challenges in studying these proteins include their uncharacterized nature and the need for advanced structural and functional analyses.
Functional Membrane Microdomains in Staphylococcus aureus
In Staphylococcus aureus, membrane proteins often organize into functional membrane microdomains (FMMs), which are crucial for bacterial nutrient acquisition and pathogenicity. Proteins like IsdF, involved in heme transport, require specific localization within these microdomains to function effectively . While SAHV_2323's role in such microdomains is speculative, understanding its potential involvement could provide insights into its function.
Data Table: General Characteristics of Recombinant UPF0060 Membrane Proteins
| Characteristic | Description |
|---|---|
| Protein Length | Typically around 100-110 amino acids |
| Expression Host | Often Escherichia coli |
| Tag | Commonly His-tagged for purification |
| Function | Potential roles in membrane organization and stability |
| Structural Analysis | Techniques include X-ray crystallography and cryo-electron microscopy |
Product Specs
Note: While we prioritize shipping the format currently in stock, please specify your preferred format in order notes for customized fulfillment.
Note: Standard shipping includes blue ice packs. Dry ice shipping requires prior arrangement and incurs additional charges.
The tag type will be determined during the production process. If you require a specific tag type, please inform us; we will prioritize development to meet your needs.
Target Background
KEGG: saw:SAHV_2323
