Recombinant Staphylococcus aureus UPF0344 protein SAHV_0964 (SAHV_0964)
Description
Molecular and Functional Characteristics
SAHV_0964 is classified as a UPF0344 family protein, though its exact biological function remains uncharacterized in current literature. Key features include:
The protein contains a conserved domain architecture typical of uncharacterized bacterial proteins, with potential transmembrane regions inferred from its hydrophobic amino acid distribution .
Production and Quality Control
Produced via recombinant expression systems (likely E. coli), SAHV_0964 undergoes rigorous purification:
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Purity: >90% (SDS-PAGE verified)
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Storage: Tris-based buffer with 50% glycerol at -20°C; avoid repeated freeze-thaw cycles .
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Applications: Primarily used as an antigen in ELISA to detect S. aureus-specific antibodies or study immune responses .
3.1. Vaccine Development
Adjuvanted recombinant proteins (e.g., CTS, SDD, SAS) have shown efficacy in eliciting γδ T-cell-mediated immunity against S. aureus infections . SAHV_0964 could similarly serve as a vaccine candidate if proven immunogenic.
3.2. Immune Evasion Mechanisms
Proteins like SpA (Protein A) suppress host immunity by binding immunoglobulins . SAHV_0964’s structural motifs warrant investigation into analogous immune-modulatory functions.
3.3. Diagnostic Utility
Recombinant S. aureus proteins (e.g., CHIPS) are used to study bacterial chemotaxis inhibition . SAHV_0964 may aid in developing assays to detect strain-specific antibody responses.
Future Research Priorities
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Functional Characterization: Determine SAHV_0964’s role in S. aureus virulence via knockout studies.
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Immunogenicity Testing: Evaluate its potential as a vaccine antigen in murine models.
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Structural Analysis: Resolve 3D structure to identify binding partners or enzymatic activity.
Product Specs
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Target Background
KEGG: saw:SAHV_0964
