Recombinant Staphylococcus aureus UPF0355 protein SA0372 (SA0372)
Description
Molecular Characterization
SA0372 is a 135-amino-acid protein (UniProt ID: Q7A7I6) with a molecular weight of approximately 15 kDa. The recombinant form is expressed in a baculovirus system and purified to >85% homogeneity . Key structural features include:
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Sequence: MADITVVNDT GELYNVINQK KSEGYLESEL TIISKSKLHL NDLHDSEISL ISTSGTFSDR MTKLLTGEDG EHAVLSRYNL APDELEKYKQ LILDDKMLVV AVRDKSSHKE VQEHNSAYEE IDITHFAEAS KGPKA .
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Domains: Contains a conserved UPF0355 domain of unknown function, common in bacterial proteins .
Functional Insights
While SA0372 remains largely uncharacterized, transcriptomic studies link it to metabolic pathways regulated by the eukaryotic-type serine/threonine kinase PknB :
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Transcriptional Regulation: SA0372 expression increases 1.8-fold in pknB mutant strains, suggesting indirect regulation by PknB-mediated signaling .
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Metabolic Context: PknB is implicated in purine biosynthesis and central metabolism, implying SA0372 might participate in stress adaptation or nutrient sensing .
Research Applications
Recombinant SA0372 is utilized in:
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Antigen Production: For antibody generation to study S. aureus proteomics.
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Protein Interaction Studies: To identify binding partners and elucidate its role in bacterial physiology.
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Structural Biology: Crystallization efforts to resolve its 3D architecture .
Table 2: Transcriptomic Profile of SA0372 in pknB Mutants
| Gene | Fold Change | Functional Category |
|---|---|---|
| SA0372 | 1.8× | Hypothetical proteins/unknown |
Ongoing Research Gaps
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Mechanistic Role: No direct evidence yet ties SA0372 to specific biochemical pathways.
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Pathogenicity Link: Its contribution to S. aureus virulence or survival in hosts remains unexplored.
Product Specs
Note: While we prioritize shipping the format we currently have in stock, we can accommodate specific format requests. Please indicate your desired format in the order notes and we will fulfill your requirements.
Note: All protein shipments are standardly packaged with blue ice packs. If dry ice packaging is required, please communicate with us in advance as additional fees will apply.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. Lyophilized forms have a shelf life of 12 months at -20°C/-80°C.
