Recombinant Stenotrophomonas maltophilia Queuine tRNA-ribosyltransferase (tgt)
Shipped with Ice Packs 
In Stock 
Description
Relevance to S. maltophilia Biology
While the provided literature does not directly address recombinant S. maltophilia TGT, insights can be inferred from related systems:
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Antibacterial Competitiveness:
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Antibiotic Resistance:
Research Gaps and Future Directions
The provided materials lack direct studies on recombinant S. maltophilia TGT. Key unresolved questions include:
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Functional Characterization:
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Substrate specificity, kinetic parameters, and structural details of the S. maltophilia enzyme.
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Role in Pathogenesis:
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Potential contributions to virulence, biofilm formation, or host immune evasion.
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Comparative Analysis of TGT Homologs
Product Specs
Form
Lyophilized powder. We will preferentially ship the format we have in stock. If you have specific format requirements, please note them when ordering, and we will fulfill your request.
Lead Time
Delivery times vary depending on the purchasing method and location. Please consult your local distributor for specific delivery times. All proteins are shipped with standard blue ice packs. For dry ice shipping, please contact us in advance; additional charges will apply.
Notes
Avoid repeated freezing and thawing. Store working aliquots at 4°C for up to one week.
Reconstitution
Briefly centrifuge the vial before opening to collect the contents at the bottom. Reconstitute the protein in sterile deionized water to a concentration of 0.1-1.0 mg/mL. We recommend adding 5-50% glycerol (final concentration) and aliquoting for long-term storage at -20°C/-80°C. Our default final glycerol concentration is 50% for your reference.
Shelf Life
Shelf life depends on several factors, including storage conditions, buffer components, storage temperature, and protein stability. Generally, the liquid form has a shelf life of 6 months at -20°C/-80°C, while the lyophilized form has a shelf life of 12 months at -20°C/-80°C.
Storage Condition
Store at -20°C/-80°C upon receipt. Aliquot for multiple uses. Avoid repeated freeze-thaw cycles.
Tag Info
The tag type will be determined during the manufacturing process. If you have a specific tag type requirement, please inform us, and we will prioritize developing the specified tag.
Synonyms
tgt; Smlt2009; Queuine tRNA-ribosyltransferase; EC 2.4.2.29; Guanine insertion enzyme; tRNA-guanine transglycosylase
Buffer Before Lyophilization
Tris/PBS-based buffer, 6%
Trehalose.
Datasheet
Please contact us to get it.
Expression Region
1-376
Protein Length
full length protein
Purity
>85% (SDS-PAGE)
Species
Stenotrophomonas maltophilia (strain K279a)
Target Names
tgt
Target Protein Sequence
MSRLQFQLQT RDGRARRGRL TFPRGTVETP AFMPVGTYGS VKGILPDDVR ALGAEIILGN
TFHLYLRPGL DIIADHGGLH GFCRWDGPIL TDSGGFQVFS LAHRRKITEQ GVTFASPTDG
ARVFLGPEES MKIQKVLDSD VVMIFDECTP YPATEDVARR SMELSLRWAQ RSRNAHDELG
NDAALFGIVQ GGVHTDLRSR SAEALQAIGF DGYAVGGLAV GEPEHERNAM LDHLDPELPS
DRPRYLMGVG RPEDLVEGVA RGVDMFDCVM PTRNARNGHY FTSFGTVRIR NSQYARDMDP
IEPGCGCVAC TGGYTRSYLR HLDRCNEMLA PMLGTLHNLF YYEKLMADIR AAIEAGTFLA
FRESFYAARG AVAPPL
Uniprot No.
Target Background
Function
Queuine tRNA-ribosyltransferase (tgt) catalyzes the replacement of guanine (G) with the queuine precursor 7-aminomethyl-7-deazaguanine (PreQ1) at position 34 (anticodon wobble position) in tRNAs with GU(N) anticodons (tRNA-Asp, -Asn, -His, and -Tyr). This occurs via a double-displacement mechanism. The active site nucleophile attacks the C1' of nucleotide 34, removing guanine and forming a covalent enzyme-RNA intermediate. The active site proton acceptor deprotonates PreQ1, enabling it to attack the C1' of the ribose and form the product. After dissociation, two further enzymatic reactions convert PreQ1 to queuine (Q), creating the hypermodified nucleoside queuosine (7-(((4,5-cis-dihydroxy-2-cyclopenten-1-yl)amino)methyl)-7-deazaguanosine).
Database Links
KEGG: sml:Smlt2009
STRING: 522373.Smlt2009
Protein Families
Queuine tRNA-ribosyltransferase family
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