Recombinant Streptococcus pyogenes serotype M49 Thymidylate kinase (tmk)

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Cat. No.
BT46668
Source
Yeast
Synonyms
tmk; Spy49_0326; Thymidylate kinase; EC 2.7.4.9; dTMP kinase
Purity
>85% (SDS-PAGE)
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Cat. No.
BT46668
Source
E.coli
Synonyms
tmk; Spy49_0326; Thymidylate kinase; EC 2.7.4.9; dTMP kinase
Purity
>85% (SDS-PAGE)
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Cat. No.
BT46668
Source
E.coli
Synonyms
tmk; Spy49_0326; Thymidylate kinase; EC 2.7.4.9; dTMP kinase
Purity
>85% (SDS-PAGE)
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Cat. No.
BT46668
Source
Baculovirus
Synonyms
tmk; Spy49_0326; Thymidylate kinase; EC 2.7.4.9; dTMP kinase
Purity
>85% (SDS-PAGE)
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Cat. No.
BT46668
Source
Mammalian cell
Synonyms
tmk; Spy49_0326; Thymidylate kinase; EC 2.7.4.9; dTMP kinase
Purity
>85% (SDS-PAGE)
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Description

Context of Thymidylate Kinase in Bacterial Systems

Thymidylate kinase (tmk) is a conserved enzyme critical for DNA synthesis, catalyzing the phosphorylation of thymidine monophosphate (dTMP) to thymidine diphosphate (dTDP). In bacterial pathogens like S. pyogenes, nucleotide biosynthesis pathways are essential for replication and virulence. While tmk is not highlighted in the provided studies, homologs in other streptococci are known to interact with metabolic and regulatory networks .

Recombinant Protein Production in S. pyogenes M49

The production of recombinant proteins in S. pyogenes M49 often involves genetic manipulation techniques such as plasmid-based deletion/insertion systems (e.g., pUC18Erm1, pFW11) . For example:

  • GPSB Recombinant Protein: Source describes a recombinant protein (UniProt ID: B5XMJ7) from S. pyogenes M49, though its annotation does not specify tmk.

ParameterDescription
Protein NameGPSB Recombinant Protein (UniProt ID: B5XMJ7)
SequenceMTSIIYSPKDIFEQEFKTSMRGFDKKEVDEFLDNVIKDYENFNAQIEALKAENEALKKAKYQARNTVSATVQQPVPQPTRVAQSATNFDILKRISKLEKEVFGKQIIE
ApplicationsWB, ELISA
Purity>85% (SDS-PAGE)
StorageTris-based buffer, 50% glycerol

Functional Analogues and Regulatory Pathways

Although tmk is not discussed, other kinases and metabolic regulators in S. pyogenes M49 provide context:

  • SP-TyK: A dual-specificity tyrosine kinase regulating cell division, virulence, and phosphorylation of CovR/WalR .

  • MsmR: Transcriptional regulator influencing metabolic and virulence genes .

  • Nudix Hydrolase: A nucleotide-metabolizing enzyme specific to M49/M82 strains, linked to survival in variable environments .

Research Gaps and Recommendations

To study recombinant tmk in S. pyogenes M49:

  1. Genomic Analysis: Query the M49 genome (e.g., strain NZ131 ) for tmk homologs (gene ID: spy_xxxx).

  2. Cloning Strategies: Use plasmids like pUC18Erm1 or pFW11 for gene knockout/complementation.

  3. Functional Assays: Assess enzymatic activity via phosphorylation assays (e.g., TLC or HPLC) .

Key Challenges

  • Annotation Ambiguity: Proteins like GPSB or SP-TyK may have undocumented roles in nucleotide metabolism.

  • Technical Limitations: Low protein yield or instability, as noted for other recombinant streptococcal proteins .

Product Specs

Form
Lyophilized powder. We will ship the in-stock format, but if you have special format requirements, please note them when ordering, and we will fulfill your request.
Lead Time
Delivery times vary based on purchase method and location. Consult your local distributor for specific delivery times. All proteins are shipped with blue ice packs by default. Request dry ice in advance for an extra fee.
Notes
Avoid repeated freeze-thaw cycles. Store working aliquots at 4°C for up to one week.
Reconstitution
Briefly centrifuge the vial before opening to collect contents. Reconstitute protein in sterile deionized water to 0.1-1.0 mg/mL. Add 5-50% glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. Our default final glycerol concentration is 50%.
Shelf Life
Shelf life depends on storage conditions, buffer components, storage temperature, and protein stability. Liquid form is generally stable for 6 months at -20°C/-80°C. Lyophilized form is generally stable for 12 months at -20°C/-80°C.
Storage Condition
Store at -20°C/-80°C upon receipt. Aliquot for multiple uses. Avoid repeated freeze-thaw cycles.
Tag Info
The tag type will be determined during production. If you require a specific tag, please inform us, and we will prioritize developing it.
Synonyms
tmk; Spy49_0326; Thymidylate kinase; EC 2.7.4.9; dTMP kinase
Buffer Before Lyophilization
Tris/PBS-based buffer, 6% Trehalose.
Datasheet
Please contact us to get it.
Expression Region
1-211
Protein Length
full length protein
Purity
>85% (SDS-PAGE)
Species
Streptococcus pyogenes serotype M49 (strain NZ131)
Target Names
tmk
Target Protein Sequence
MITGKLITVE GPDGAGKTTV LEQLIPLLKQ KVAQDILTTR EPGGVAISEH IRELILDINH TAMDPKTELL LYIAARRQHL VEKVLPALEA GQLVFIDRFI DSSVAYQGAG RGLTKADIQW LNEFATDGLE PDLTLYFDVP SEIGLARINA NQQREINRLD LETIEIHQRV RKGYLALAKE HPKRIVTIDA TKPLKEVVSV ALEHVLALLL A
Uniprot No.
B5XK00

Target Background

Function
Phosphorylates dTMP to dTDP in both de novo and salvage pathways of dTTP synthesis.
Database Links

KEGG: soz:Spy49_0326

Protein Families
Thymidylate kinase family

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