Recombinant Streptomyces griseus subsp. griseus Argininosuccinate synthase (argG)

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Cat. No.
BT41403
Source
Yeast
Synonyms
argG; SGR_5966Argininosuccinate synthase; EC 6.3.4.5; Citrulline--aspartate ligase
Purity
>85% (SDS-PAGE)
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Cat. No.
BT41403
Source
E.coli
Synonyms
argG; SGR_5966Argininosuccinate synthase; EC 6.3.4.5; Citrulline--aspartate ligase
Purity
>85% (SDS-PAGE)
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Cat. No.
BT41403
Source
E.coli
Synonyms
argG; SGR_5966Argininosuccinate synthase; EC 6.3.4.5; Citrulline--aspartate ligase
Purity
>85% (SDS-PAGE)
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Cat. No.
BT41403
Source
Baculovirus
Synonyms
argG; SGR_5966Argininosuccinate synthase; EC 6.3.4.5; Citrulline--aspartate ligase
Purity
>85% (SDS-PAGE)
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Cat. No.
BT41403
Source
Mammalian cell
Synonyms
argG; SGR_5966Argininosuccinate synthase; EC 6.3.4.5; Citrulline--aspartate ligase
Purity
>85% (SDS-PAGE)
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Description

Gene Structure and Recombinant Expression

The argG gene encodes a 56-kDa protein with a conserved catalytic domain. In Streptomyces species, argG is often cloned using hybridization probes or PCR amplification. Key features include:

FeatureDescriptionSource
Gene Length~1449 bp (encoding 483 amino acids)
Protein Size~56 kDa
Catalytic ActivitySynthesizes argininosuccinate from citrulline and ATP
Expression HostE. coli, Lactobacillus plantarum (heterologous systems)
Purity>80% by SDS-PAGE (recombinant protein)

Note: While Streptomyces griseus subsp. griseus is not directly cited in argG studies, the gene’s structural conservation across Streptomyces species suggests similar functional properties.

Functional Characterization

Recombinant argG has been studied in heterologous hosts to evaluate its role in acid tolerance and metabolic pathways:

Enzyme Activity

In Lactobacillus plantarum, heterologous expression of argG (from Oenococcus oeni) significantly enhanced argininosuccinate synthase (ASS) activity under acidic conditions:

ParameterControl Strain (pMG36e)Recombinant Strain (pMG36e-argG)Fold Increase
ASS Activity (pH 3.7)Undetectable11× higher than control (pH 6.3)260%
Arginine LevelsBasalElevated under acid stressN/A

Source: Modified from

Acid Stress Response

The recombinant argG strain exhibited improved survival at pH 3.7, attributed to:

  • Upregulated ADI Pathway: Increased expression of argG, argH, and argF genes.

  • Metabolic Shifts: Enhanced aspartate, glutamate, and arginine production.

  • pH Homeostasis: Elevated intracellular ATP and H⁺-ATPase activity.

Industrial Microbiology

  • Acid-Tolerant Strains: Recombinant argG enhances microbial survival in acidic environments, relevant for food fermentation (e.g., wine, dairy).

  • Bioprocessing: ASS activity could modulate arginine production for biotechnological applications.

Research Implications

  • Gene Regulation: argG expression is induced under acid stress, suggesting potential roles in pH adaptation.

  • Cross-Species Utility: Insights from O. oeni and L. plantarum systems may inform engineering of Streptomyces strains.

Limitations and Future Directions

  1. Direct Studies on S. griseus subsp. griseus: No peer-reviewed data explicitly addresses argG in this subspecies.

  2. Host-Specific Optimization: Expression efficiency may vary in Streptomyces vs. heterologous hosts.

  3. Mechanistic Studies: Further research is needed to elucidate argG’s role in Streptomyces secondary metabolism.

Product Specs

Form
Lyophilized powder. We will preferentially ship the available format. If you have specific format requirements, please note them when ordering.
Lead Time
Delivery times vary based on purchasing method and location. Consult your local distributor for specific delivery times. All proteins are shipped with blue ice packs by default. Requesting dry ice will incur extra fees; please communicate this in advance.
Notes
Avoid repeated freeze-thaw cycles. Working aliquots can be stored at 4°C for up to one week.
Reconstitution
Briefly centrifuge the vial before opening. Reconstitute the protein in sterile deionized water to 0.1-1.0 mg/mL. Add 5-50% glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. Our default final glycerol concentration is 50%.
Shelf Life
Shelf life depends on storage conditions, buffer components, temperature, and protein stability. Generally, the liquid form is stable for 6 months at -20°C/-80°C, and the lyophilized form is stable for 12 months at -20°C/-80°C.
Storage Condition
Store at -20°C/-80°C upon receipt. Aliquot for multiple uses. Avoid repeated freeze-thaw cycles.
Tag Info
The tag type is determined during manufacturing. If you require a specific tag, please inform us, and we will prioritize its development.
Synonyms
argG; SGR_5966Argininosuccinate synthase; EC 6.3.4.5; Citrulline--aspartate ligase
Buffer Before Lyophilization
Tris/PBS-based buffer, 6% Trehalose.
Datasheet
Please contact us to get it.
Expression Region
1-397
Protein Length
full length protein
Purity
>85% (SDS-PAGE)
Species
Streptomyces griseus subsp. griseus (strain JCM 4626 / NBRC 13350)
Target Names
argG
Target Protein Sequence
MTERVVLAYS GGLDTSVAIG WIAEETGAEV IAVAVDVGQG GEDLDVIRKR ALACGAVEAE VADARDEFAE EYCLPAIKAN ALYMDRYPLV SALSRPTIVK HLVAAARKHG AGTVAHGCTG KGNDQVRFEA GISALGPDLK CIAPVRDYAM TRDRAIAFCE EKNLPIATTR KSPYSIDQNV FGRAVETGFL EDIWNAPIED IYEYTADPAV PREADEVVIS FKEGVPVAVD GRPVTVLQAI QQLNERAGAQ GVGRIDMVED RLVGIKSREV YEAPGAIALI TAHQELENVT VERELARYKR QVEQRWGEMV YDGLWFSPLK RALDGFINEA NQHVTGDIRM TLHAGRAVVT GRKSEESLYD FNLATYDSGD TFDQSKAQGF IEIFGLSAKI AARRDLA
Uniprot No.
B1W3B3

Target Background

Database Links

KEGG: sgr:SGR_5966

STRING: 455632.SGR_5966

Protein Families
Argininosuccinate synthase family, Type 1 subfamily
Subcellular Location
Cytoplasm.

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