Recombinant Streptomyces griseus subsp. griseus tRNA dimethylallyltransferase (miaA)
Shipped with Ice Packs 
In Stock 
Product Specs
Form
Lyophilized powder. We will ship the in-stock format preferentially. If you have specific format requirements, please note them during order placement.
Lead Time
Delivery times vary based on purchasing method and location. Consult your local distributor for specific delivery times. All proteins are shipped with standard blue ice packs. For dry ice shipping, contact us in advance; additional fees apply.
Notes
Avoid repeated freeze-thaw cycles. Working aliquots are stable at 4°C for up to one week.
Reconstitution
Briefly centrifuge the vial before opening. Reconstitute the protein in sterile deionized water to a concentration of 0.1-1.0 mg/mL. Adding 5-50% glycerol (final concentration) is recommended for long-term storage at -20°C/-80°C. Our default final glycerol concentration is 50%.
Shelf Life
Shelf life depends on storage conditions, buffer components, temperature, and protein stability. Liquid form is generally stable for 6 months at -20°C/-80°C. Lyophilized form is generally stable for 12 months at -20°C/-80°C.
Storage Condition
Store at -20°C/-80°C upon receipt. Aliquot for multiple uses. Avoid repeated freeze-thaw cycles.
Tag Info
Tag type is determined during manufacturing. If you require a specific tag, please inform us, and we will prioritize its development.
Synonyms
miaA; SGR_1729tRNA dimethylallyltransferase; EC 2.5.1.75; Dimethylallyl diphosphate:tRNA dimethylallyltransferase; DMAPP:tRNA dimethylallyltransferase; DMATase; Isopentenyl-diphosphate:tRNA isopentenyltransferase; IPP transferase; IPPT; IPTase
Buffer Before Lyophilization
Tris/PBS-based buffer, 6%
Trehalose.
Datasheet
Please contact us to get it.
Expression Region
1-312
Protein Length
full length protein
Purity
>85% (SDS-PAGE)
Species
Streptomyces griseus subsp. griseus (strain JCM 4626 / NBRC 13350)
Target Names
miaA
Target Protein Sequence
MTPPASAPRV ITVVGPTAAG KSDLGVFLAQ RLGGEVINAD SMQLYRGMDI GTAKLTLPER
EGVPHRLLDI WDVTETASVA EYQRLARREI DRLLAEGRTP ILVGGSGLYV KGAIDALEFP
GTDPEVRARL EAELAERGSG VLHERLAAAD PDAARSILAS NGRRIVRALE VIEITGKPFT
ANLPGDEPVY DAVQIGVDVE RPELDERIAR RVDRMWDAGL VDEVRALEAA GLREGLTASR
ALGYQQILAV LAGECTEDDA RAETVRATKR FARRQDSWFR RDARVVWLGG GHRDRGELPH
RALTLVERAV TA
Uniprot No.
Target Background
Function
Catalyzes the transfer of a dimethylallyl group to the adenine at position 37 in tRNAs that read codons beginning with uridine, forming N6-(dimethylallyl)adenosine (i(6)A).
Database Links
KEGG: sgr:SGR_1729
STRING: 455632.SGR_1729
Protein Families
IPP transferase family
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