Recombinant Streptomyces griseus subsp. griseus Undecaprenyl-diphosphatase (uppP)
Description
Recombinant UppP Production in Model Systems
While no studies on S. griseus UppP were identified, recombinant UppP from Azospirillum brasilense (UniProt ID: P39438) has been expressed in E. coli with a His-tag . Key parameters from this homolog include:
| Parameter | Details |
|---|---|
| Expression Host | E. coli |
| Tag | N-terminal His tag |
| Protein Length | 187 amino acids (full-length) |
| Purity | >90% (SDS-PAGE verified) |
| Storage | Lyophilized in Tris/PBS buffer with 6% trehalose (pH 8.0) . |
This template could guide analogous recombinant production of S. griseus UppP.
Functional Insights from UppP Homologs
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Essentiality: In B. subtilis, UppP and BcrC form a synthetic lethal pair; depletion causes cell lysis due to UP shortage .
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Antibiotic Resistance: UppP overexpression confers bacitracin resistance in E. coli by competing for UPP binding .
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Sporulation Role: B. subtilis UppP is critical for sporulation, with mutants showing defective spore maturation .
Research Gaps and Future Directions
Despite extensive studies on UppP in E. coli and B. subtilis, no peer-reviewed data exists for S. griseus subsp. griseus recombinant UppP. Key unresolved questions:
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Does S. griseus UppP share the periplasmic orientation observed in E. coli ?
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How does its activity compare to homologs under antibiotic stress (e.g., bacitracin)?
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Can structural modeling (e.g., Rosetta membrane ab initio ) predict its catalytic mechanism?
Industrial and Therapeutic Implications
Streptomyces griseus is a prolific antibiotic producer , and UppP inhibitors (e.g., bacitracin analogs) could synergize with existing cell wall-targeting drugs . Recombinant UppP studies could facilitate:
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High-throughput screening for novel phosphatase inhibitors.
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Engineering bacitracin-resistant strains for industrial applications.
Product Specs
Note: While we prioritize shipping the format currently in stock, please specify your format preference during order placement for customized preparation.
Note: All proteins are shipped with standard blue ice packs. Dry ice shipping requires prior arrangement and incurs additional charges.
The tag type is determined during production. If you require a specific tag, please inform us; we will prioritize development accordingly.
Target Background
KEGG: sgr:SGR_6235
STRING: 455632.SGR_6235
