Recombinant Synechococcus sp. Translation initiation factor IF-2 (infB), partial
Shipped with Ice Packs 
In Stock 
Description
Functional Role in Translation Initiation
IF2 orchestrates early steps of protein synthesis:
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Subunit Association: Binds the 30S and 50S ribosomal subunits, facilitating 70S complex formation .
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fMet-tRNA Recruitment: Positions fMet-tRNA in the ribosomal P-site via its C-terminal domain .
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GTP Hydrolysis: Triggers conformational changes in the ribosome, releasing IF2 and transitioning to elongation .
Comparative Analysis of Bacterial IF2
Applications and Research Gaps
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Biotechnological Use: Recombinant IF2 fragments enable studies on translation mechanisms in cyanobacteria, which are critical for bioengineering photosynthetic organisms .
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Unresolved Questions:
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How do Synechococcus IF2 isoforms differ in function?
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Does phage-derived HGT influence infB evolution in marine cyanobacteria?
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Product Specs
Form
Lyophilized powder. We will ship the in-stock format by default. If you have specific format requirements, please note them when ordering.
Lead Time
Delivery times vary by purchase method and location. Contact your local distributor for specific delivery information. All proteins are shipped with standard blue ice packs. For dry ice shipping, please contact us in advance; additional fees apply.
Notes
Avoid repeated freezing and thawing. Working aliquots are stable at 4°C for up to one week.
Reconstitution
Briefly centrifuge the vial before opening. Reconstitute the protein in sterile deionized water to 0.1-1.0 mg/mL. Add 5-50% glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. The default final glycerol concentration is 50%.
Shelf Life
Shelf life depends on storage conditions, buffer components, storage temperature, and protein stability. Liquid form is generally stable for 6 months at -20°C/-80°C. Lyophilized form is generally stable for 12 months at -20°C/-80°C.
Storage Condition
Store at -20°C/-80°C upon receipt. Aliquot for multiple uses. Avoid repeated freeze-thaw cycles.
Tag Info
The tag type is determined during manufacturing. If you require a specific tag, please inform us, and we will prioritize its development.
Synonyms
infB; SynRCC307_1970Translation initiation factor IF-2
Buffer Before Lyophilization
Tris/PBS-based buffer, 6%
Trehalose.
Datasheet
Please contact us to get it.
Protein Length
Partial
Purity
>85% (SDS-PAGE)
Species
Synechococcus sp. (strain RCC307)
Target Names
infB
Uniprot No.
Target Background
Function
Essential for initiating protein synthesis. Protects formylmethionyl-tRNA from hydrolysis and promotes its binding to the 30S ribosomal subunit. Also involved in GTP hydrolysis during 70S ribosomal complex formation.
Database Links
KEGG: syr:SynRCC307_1970
STRING: 316278.SynRCC307_1970
Protein Families
TRAFAC class translation factor GTPase superfamily, Classic translation factor GTPase family, IF-2 subfamily
Subcellular Location
Cytoplasm.
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