Recombinant Synechocystis sp. 30S ribosomal protein S15 (rpsO)

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Cat. No.
BT38036
Source
Yeast
Synonyms
rpsO; rps15; ssl1784; 30S ribosomal protein S15
Purity
>85% (SDS-PAGE)
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Cat. No.
BT38036
Source
E.coli
Synonyms
rpsO; rps15; ssl1784; 30S ribosomal protein S15
Purity
>85% (SDS-PAGE)
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Cat. No.
BT38036
Source
E.coli
Synonyms
rpsO; rps15; ssl1784; 30S ribosomal protein S15
Purity
>85% (SDS-PAGE)
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Cat. No.
BT38036
Source
Baculovirus
Synonyms
rpsO; rps15; ssl1784; 30S ribosomal protein S15
Purity
>85% (SDS-PAGE)
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Cat. No.
BT38036
Source
Mammalian cell
Synonyms
rpsO; rps15; ssl1784; 30S ribosomal protein S15
Purity
>85% (SDS-PAGE)
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Description

Overview of Ribosomal Protein S15

Ribosomal protein S15 is a primary binding protein critical for 30S subunit assembly. In E. coli, S15 orchestrates the hierarchical integration of proteins S6, S11, S18, and S21 with the central domain of 16S rRNA, forming the platform of the 30S subunit . Key findings include:

  • Role in Assembly: S15 binds directly to 16S rRNA, enabling secondary protein assembly and ensuring structural integrity of the 30S subunit .

  • Functional Plasticity: E. coli strains lacking S15 (ΔrpsO) remain viable but exhibit impaired ribosome biogenesis, cold sensitivity, and defective subunit association .

Synechocystis Ribosomal Proteins and Genetic Context

While Synechocystis sp. PCC 6803 has been extensively studied for exopolysaccharide (EPS) biosynthesis and stress responses , ribosomal protein S15 (rpsO) has not been explicitly characterized in this organism. Relevant observations include:

  • Genomic Redundancy: Synechocystis exhibits genetic redundancy in pathways like EPS export (e.g., three kpsM homologs ), suggesting potential compensatory mechanisms in ribosomal assembly.

  • CRISPRi Applications: Recent studies used CRISPR interference (CRISPRi) to repress multiple genes in Synechocystis, highlighting methodologies that could be adapted to study ribosomal proteins .

Hypothetical Function of Recombinant S15 in Synechocystis

Based on E. coli data , recombinant S15 in Synechocystis could theoretically serve roles in:

  • 30S Subunit Assembly: Stabilizing rRNA-protein interactions during ribosome biogenesis.

  • Subunit Association: Facilitating 70S ribosome formation through interface bridging.

  • Stress Adaptation: Potential involvement in cold tolerance, as seen in E. coli ΔrpsO strains .

Research Gaps and Future Directions

AspectCurrent KnowledgeUnanswered Questions
S15 StructureKnown in E. coli 3D conformation in Synechocystis
Recombinant ExpressionNot reportedOptimal expression systems (e.g., vectors)
Functional RedundancyObserved in EPS pathways Compensatory mechanisms for S15 absence

Product Specs

Form
Lyophilized powder. We will ship the available format. If you have specific format requirements, please note them during order placement.
Lead Time
Delivery time varies based on purchasing method and location. Contact your local distributor for specific delivery information. All proteins are shipped with standard blue ice packs. Request dry ice shipment in advance (extra fees apply).
Notes
Avoid repeated freezing and thawing. Store working aliquots at 4°C for up to one week.
Reconstitution
Briefly centrifuge the vial before opening. Reconstitute protein in sterile deionized water to 0.1-1.0 mg/mL. Add 5-50% glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. Our default final glycerol concentration is 50% for reference.
Shelf Life
Shelf life depends on storage conditions, buffer components, storage temperature, and protein stability. Liquid form: 6 months at -20°C/-80°C. Lyophilized form: 12 months at -20°C/-80°C.
Storage Condition
Store at -20°C/-80°C upon receipt. Aliquot for multiple uses. Avoid repeated freeze-thaw cycles.
Tag Info
Tag type is determined during manufacturing. If you require a specific tag, please inform us, and we will prioritize its development.
Synonyms
rpsO; rps15; ssl1784; 30S ribosomal protein S15
Buffer Before Lyophilization
Tris/PBS-based buffer, 6% Trehalose.
Datasheet
Please contact us to get it.
Expression Region
1-89
Protein Length
full length protein
Purity
>85% (SDS-PAGE)
Species
Synechocystis sp. (strain PCC 6803 / Kazusa)
Target Names
rpsO
Target Protein Sequence
MSLTQIRKQE LMTEYQAHET DTGSADLQVA FLTERITQLT GHLKANPKDH ASRRGLLKMI GRRKRLLSFI NAREPERYQA LIKRLGIRR
Uniprot No.
P72866

Target Background

Function
S15 is a primary rRNA binding protein that binds directly to 16S rRNA. It helps initiate 30S subunit assembly by binding and bridging several RNA helices. It forms intersubunit bridge B4 with the 50S subunit's 23S rRNA.
Database Links

KEGG: syn:ssl1784

STRING: 1148.SYNGTS_0306

Protein Families
Universal ribosomal protein uS15 family

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