Recombinant Synechocystis sp. 50S ribosomal protein L29 (rpmC)

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Cat. No.

BT36973

Source

Yeast

Synonyms

rpmC; rpl29; ssl3436; 50S ribosomal protein L29

Purity

>85% (SDS-PAGE)

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Cat. No.

BT36973

Source

E.coli

Synonyms

rpmC; rpl29; ssl3436; 50S ribosomal protein L29

Purity

>85% (SDS-PAGE)

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Cat. No.

BT36973

Source

E.coli

Synonyms

rpmC; rpl29; ssl3436; 50S ribosomal protein L29

Purity

>85% (SDS-PAGE)

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Cat. No.

BT36973

Source

Baculovirus

Synonyms

rpmC; rpl29; ssl3436; 50S ribosomal protein L29

Purity

>85% (SDS-PAGE)

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Cat. No.

BT36973

Source

Mammalian cell

Synonyms

rpmC; rpl29; ssl3436; 50S ribosomal protein L29

Purity

>85% (SDS-PAGE)

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Description

Introduction to Recombinant Synechocystis sp. 50S Ribosomal Protein L29 (rpmC)

Recombinant Synechocystis sp. 50S ribosomal protein L29 (rpmC) is a bacterially derived ribosomal subunit protein essential for translation and ribosome assembly. This protein, encoded by the rpmC gene, forms part of the large ribosomal subunit (50S) and plays a role in translational fidelity and structural stabilization . Recombinant versions are produced for research applications, including structural studies, stress response analyses, and antibody development .

Primary Structure

Amino Acid Sequence:
The full-length protein (69 amino acids) varies slightly between Synechocystis strains. For example:
- Strain WH7803:
  MARPNASELR QLSDADITEQ INGLRRELFD LRFQQATRQL GNTHRFKQSR IKLAQLLTVQ KERQSSTAS
- Strain WH8102:
  MARPNAAEVR QLSDTDITEQ INGLRRELFQ LRFQQATRQL ANTHRFKEVR IKLAQLMTVQ SERQRSAAS
Molecular Weight:
- Calculated: ~18 kDa (varies by post-translational modifications)
- Observed: 20–25 kDa (SDS-PAGE)

Role in Stress Response

Low Salinity Stress:
In Prochlorococcus sp. strain NATL1A, rpmC expression decreased significantly under low salinity (log₂FC = -0.752), suggesting ribosomal remodeling during osmotic stress .
Light Irradiation:
Synechocystis sp. PCC 6803 showed differential expression of ribosomal proteins, including L29, under UV-B and high-intensity light, indicating light-regulated translational adjustments .

Pathological Relevance

RPL29 is upregulated in human carcinomas and serves as a biomarker for abnormal cell growth .

Antibody-Based Detection

Application	Dilution Range	Recommended Use
Western Blot	1:5,000–1:50,000	Detects RPL29 in HeLa, HepG2, and NIH/3T3 cells
Immunofluorescence	1:200–1:800	Localization in HepG2 cells
Flow Cytometry	0.25 µg/10⁶ cells	Intracellular staining

Product Specs

Form

Lyophilized powder. We will ship the format in stock, but if you have special format requirements, please note them when ordering, and we will fulfill your request.

Lead Time

Delivery times vary based on purchase method and location. Contact your local distributor for specific delivery times. All proteins are shipped with standard blue ice packs. For dry ice shipping, please contact us in advance; extra fees apply.

Notes

Avoid repeated freezing and thawing. Store working aliquots at 4°C for up to one week.

Reconstitution

Briefly centrifuge the vial before opening to collect contents at the bottom. Reconstitute the protein in sterile deionized water to 0.1-1.0 mg/mL. Add 5-50% glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. Our default final glycerol concentration is 50%.

Shelf Life

Shelf life depends on storage conditions, buffer components, storage temperature, and protein stability. Liquid form shelf life is generally 6 months at -20°C/-80°C. Lyophilized form shelf life is generally 12 months at -20°C/-80°C.

Storage Condition

Store at -20°C/-80°C upon receiving. Aliquot for multiple uses. Avoid repeated freeze-thaw cycles.

Tag Info

Tag type is determined during manufacturing. If you have a specific tag type requirement, please inform us, and we will prioritize developing it.

Synonyms

rpmC; rpl29; ssl3436; 50S ribosomal protein L29

Buffer Before Lyophilization

Tris/PBS-based buffer, 6% Trehalose.

Datasheet

Please contact us to get it.

Expression Region

1-73

Protein Length

full length protein

Purity

>85% (SDS-PAGE)

Species

Synechocystis sp. (strain PCC 6803 / Kazusa)

Target Names

rpmC

Target Protein Sequence

MALPNIADAR KLGDEELATE ILATKQRLFQ LRFQQATRRP ENPHEFKHAR HRLAQLLTVE RERQLENSPS EEA

Uniprot No.

P73312

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Recombinant Synechocystis sp. 50S ribosomal protein L29 (rpmC)

Description

Introduction to Recombinant Synechocystis sp. 50S Ribosomal Protein L29 (rpmC)

Primary Structure

Role in Stress Response

Pathological Relevance

Antibody-Based Detection

Product Specs

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