Recombinant Synechocystis sp. Aspartyl/glutamyl-tRNA (Asn/Gln) amidotransferase subunit B

Shipped with Ice Packs
In Stock
Cat. No.
BT43048
Source
Yeast
Synonyms
gatB; sll1435; Aspartyl/glutamyl-tRNA(Asn/Gln) amidotransferase subunit B; Asp/Glu-ADT subunit B; EC 6.3.5.-
Purity
>85% (SDS-PAGE)
Quick Inquiry
Cat. No.
BT43048
Source
E.coli
Synonyms
gatB; sll1435; Aspartyl/glutamyl-tRNA(Asn/Gln) amidotransferase subunit B; Asp/Glu-ADT subunit B; EC 6.3.5.-
Purity
>85% (SDS-PAGE)
Quick Inquiry
Cat. No.
BT43048
Source
E.coli
Synonyms
gatB; sll1435; Aspartyl/glutamyl-tRNA(Asn/Gln) amidotransferase subunit B; Asp/Glu-ADT subunit B; EC 6.3.5.-
Purity
>85% (SDS-PAGE)
Quick Inquiry
Cat. No.
BT43048
Source
Baculovirus
Synonyms
gatB; sll1435; Aspartyl/glutamyl-tRNA(Asn/Gln) amidotransferase subunit B; Asp/Glu-ADT subunit B; EC 6.3.5.-
Purity
>85% (SDS-PAGE)
Quick Inquiry
Cat. No.
BT43048
Source
Mammalian cell
Synonyms
gatB; sll1435; Aspartyl/glutamyl-tRNA(Asn/Gln) amidotransferase subunit B; Asp/Glu-ADT subunit B; EC 6.3.5.-
Purity
>85% (SDS-PAGE)
Quick Inquiry

Description

Functional Role in tRNA Transamidation

GatB operates in the GatABC complex to rectify tRNA mischarging:

  • Mechanism: Collaborates with GatA and GatC to hydrolyze misacylated Glu-tRNAGln or Asp-tRNAAsn, transferring the amide group from glutamine to form correctly charged Gln-tRNAGln or Asn-tRNAAsn .

  • Zinc Dependence: Activity is modulated by zinc availability; homologs in other bacteria (e.g., Anabaena) show altered oligomerization under zinc-limiting conditions .

Production and Biotechnological Applications

Recombinant GatB is produced in E. coli expression systems for research use:

Research Applications

  • Enzyme Kinetics: Used to study transamidation rates and substrate specificity .

  • Structural Biology: Facilitates crystallography studies to map catalytic residues .

  • Metabolic Engineering: Explored in cyanobacterial models for optimizing nitrogen metabolism .

Comparative Insights from Related Studies

  • Complex Stability: In Helicobacter pylori, GatB forms membrane-associated complexes with adhesins (e.g., BabA), suggesting moonlighting roles beyond translation .

  • Oligomerization: Duplicated aminoacyl-tRNA synthetases (aaRSs) in Anabaena exhibit trans-oligomerization, a mechanism proposed to extend to GatB homologs under stress .

Challenges and Future Directions

  • Orphan Functions: Over 30% of Synechocystis proteins remain uncharacterized; GatB interaction networks (e.g., with GatA/C) require deeper exploration .

  • Biomedical Potential: GatB’s role in bacterial viability makes it a candidate for antimicrobial drug targeting .

Product Specs

Form
Lyophilized powder. We will ship the available format, but if you have specific requirements, please note them when ordering, and we will fulfill your request.
Lead Time
Delivery time varies based on purchasing method and location. Please consult your local distributor for specific delivery times. All proteins are shipped with standard blue ice packs. For dry ice shipping, contact us in advance; additional fees will apply.
Notes
Avoid repeated freezing and thawing. Store working aliquots at 4°C for up to one week.
Reconstitution
Briefly centrifuge the vial before opening. Reconstitute protein in sterile deionized water to 0.1-1.0 mg/mL. Add 5-50% glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. Our default final glycerol concentration is 50%.
Shelf Life
Shelf life depends on storage conditions, buffer components, storage temperature, and protein stability. Generally, the liquid form is stable for 6 months at -20°C/-80°C, while the lyophilized form is stable for 12 months at -20°C/-80°C.
Storage Condition
Store at -20°C/-80°C upon arrival. Aliquot for multiple uses. Avoid repeated freeze-thaw cycles.
Tag Info
Tag type is determined during manufacturing. If you require a specific tag, please inform us, and we will prioritize its development.
Synonyms
gatB; sll1435; Aspartyl/glutamyl-tRNA(Asn/Gln) amidotransferase subunit B; Asp/Glu-ADT subunit B; EC 6.3.5.-
Buffer Before Lyophilization
Tris/PBS-based buffer, 6% Trehalose.
Datasheet
Please contact us to get it.
Expression Region
1-495
Protein Length
full length protein
Purity
>85% (SDS-PAGE)
Species
Synechocystis sp. (strain PCC 6803 / Kazusa)
Target Names
gatB
Target Protein Sequence
MTATAVKTDY EAIIGLETHC QLNTASKIFC NCSTDFDSPP NTNVCPVCLG YPGVLPVLNE EVLAAAVKLG LAINGQIAPY SKFDRKQYFY PDLPKNYQIS QFDLPIVEHG SLEIELVDKK TKEITRKTIG ITRLHMEEDA GKLVHAGSDR LAGSTHSLVD FNRTGVPLLE IVSEPDLRTG QEAAEYAQSL RQLVRYLGIS DGNMQEGSLR CDVNISVRPV GQKEFGTKVE IKNMNSFSAI QKAIEYEIER QIKAIADGEE IYQETRLWEE GSQRTISMRK KEGSSDYRYF PEPDLPPLEV SEQQKQTWFA TLPELPSAKR ARYEQKLGLS AYDARVLTED RDVALYFEEA VNAGADPKAV ANWVTQDIAA YLNNNRLTIS GLALKPSALA ELIDLITKGT ISGKIAKDIL PELLEKGGSP QKIIEARGLV QISDPDELTA IIQAVIADHP KELEKFRSGK GNMQGFFVGQ VMKRTGGKAD PKLTNQLIGK LLAQA
Uniprot No.
P74215

Target Background

Function
Enables the formation of correctly charged Asn-tRNA(Asn) or Gln-tRNA(Gln) by converting misacylated Asp-tRNA(Asn) or Glu-tRNA(Gln) in organisms lacking asparaginyl-tRNA or glutaminyl-tRNA synthetases. This transamidation reaction requires glutamine and ATP and involves an activated phospho-Asp-tRNA(Asn) or phospho-Glu-tRNA(Gln) intermediate.
Database Links

KEGG: syn:sll1435

STRING: 1148.SYNGTS_1734

Protein Families
GatB/GatE family, GatB subfamily

Quick Inquiry

Personal Email Detected
Please use an institutional or corporate email address for inquiries. Personal email accounts ( such as Gmail, Yahoo, and Outlook) are not accepted. *

Category

Service

THE BIOTEK
THE BioTek is a global biotech company offering highly purified proteins for research in cancer, apoptosis, development, endocrinology, immunology, neuroscience, proteases, and stem cells.
  • Contact
  • Address: 1925 E Maple Ave, El Segundo, CA 90245 United States
  • Phone : +1 201-285-7826
  • Email : info@thebiotek.com
  • Hours : Mon.-Fri. 9:00 AM - 5:00 PM
© Copyright 2025 TheBiotek. All Rights Reserved.