Recombinant Synechocystis sp. ATP synthase subunit a (atpB)
Description
Structure and Function of ATP Synthase Subunit a (AtpB)
The FF ATP synthase in Synechocystis sp. PCC 6803 consists of two main sectors:
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F: Catalyzes ATP synthesis/hydrolysis via αβγ subunits.
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F: A membrane-embedded proton channel composed of subunits a (AtpB), b, and c .
Subunit a (AtpB) is essential for coupling proton movement with rotational mechanics in the F sector. Mutagenesis studies in Synechocystis have shown that modifications to other subunits (e.g., γ or ϵ) indirectly affect proton translocation efficiency and ATP synthesis rates, highlighting the interdependence of F and F components .
Key Features of Recombinant Cyanobacterial AtpB
| Property | Description | Source |
|---|---|---|
| Host System | E. coli | |
| Tag | N-terminal His tag | |
| Amino Acid Range | 1–239 aa | |
| Molecular Function | Proton channel formation, rotational coupling with F sector |
Functional Insights
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Proton Translocation: AtpB works with subunit c to create a proton gradient-driven rotation, enabling ATP synthesis .
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Regulatory Role: Interactions with γ and ϵ subunits modulate ATPase activity to prevent energy waste during dark conditions .
Physiological Impact of Subunit Modifications
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Deletion of regulatory regions in γ (e.g., β-hairpin) reduces ATP synthesis efficiency by ~30% in Synechocystis .
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Truncation of the ϵ subunit’s C-terminal domain increases ATP hydrolysis activity, lowering intracellular ATP levels during prolonged darkness .
Comparative Analysis
| Strain/Mutation | ATP Synthesis Rate | ATP Hydrolysis Activity | Survival in Darkness |
|---|---|---|---|
| Wild-Type Synechocystis | 100% | Baseline | High |
| γ Δ198–222 | ~70% | Elevated | Reduced |
| ϵ ΔC | ~85% | 2× baseline | Moderately Reduced |
These findings underscore AtpB’s role in maintaining energy homeostasis, particularly under stress conditions like nutrient limitation or darkness .
Technical Challenges and Future Directions
Product Specs
Note: While we prioritize shipping the format we have in stock, we can fulfill specific format requirements. Please indicate your desired format in the order notes, and we will prepare the product accordingly.
Note: Our proteins are shipped with standard blue ice packs. If you require dry ice shipping, please communicate this in advance, as additional charges will apply.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. Lyophilized form has a shelf life of 12 months at -20°C/-80°C.
Target Background
KEGG: syn:sll1322
STRING: 1148.SYNGTS_0164
