Recombinant Synechocystis sp. Urease accessory protein UreD (ureD)
Description
Introduction to UreD
UreD is a critical accessory protein in the maturation pathway of urease, an enzyme essential for urea hydrolysis in cyanobacteria like Synechocystis sp. PCC 6803 . As part of the UreFD complex, UreD facilitates the transfer of nickel ions (Ni²⁺) to the urease apoprotein during enzyme activation. Its structural and functional roles are central to nitrogen metabolism, particularly in environments where urea serves as a nitrogen source.
Research Findings
Table 1: Key Experimental Data on UreD Function
Mutagenesis Studies: Mutations in UreD’s tunnel residues (e.g., residues 38–86) impair nickel transfer without affecting UreD-urease binding . This underscores UreD’s dual role in structural integrity and catalytic assistance.
Applications in Biotechnology
While direct applications of recombinant UreD are not extensively reported, its role in urease maturation suggests potential utility in:
Product Specs
Target Background
KEGG: syn:sll1639
STRING: 1148.SYNGTS_0492
