Recombinant Tetraodon nigroviridis 60S ribosome subunit biogenesis protein NIP7 homolog (nip7)

Introduction to Recombinant Tetraodon nigroviridis 60S Ribosome Subunit Biogenesis Protein NIP7 Homolog

The Recombinant Tetraodon nigroviridis 60S ribosome subunit biogenesis protein NIP7 homolog (nip7) is a protein involved in the biogenesis of the 60S ribosomal subunit. While specific information on the Tetraodon nigroviridis version of this protein is limited, insights can be drawn from studies on its homologs in other organisms, particularly Saccharomyces cerevisiae (baker's yeast), where Nip7p plays a crucial role in ribosome biogenesis.

Function of NIP7 Homologs

NIP7 homologs are essential for efficient 60S ribosome subunit assembly. In Saccharomyces cerevisiae, Nip7p is required for proper pre-rRNA processing and the assembly of the 60S ribosomal subunit . This protein is not a ribosomal protein itself but interacts with free 60S subunits, suggesting its role in the maturation process of these subunits .

Evolutionary Conservation

The NIP7 protein shows evolutionary conservation across different species, including humans, Caenorhabditis elegans, and Arabidopsis thaliana . This conservation indicates that the function of NIP7 is fundamental and necessary for cellular processes across diverse organisms.

Expression and Production

Recombinant proteins like the NIP7 homolog can be expressed in various host systems, such as Escherichia coli (E. coli), HEK-293 cells, or cell-free protein synthesis systems . These systems allow for the production of high-purity proteins for research and potential therapeutic applications.

Saccharomyces cerevisiae Nip7p

• Phenotypes of Nip7p Depletion: Cells depleted of Nip7p exhibit hypersensitivity to paromomycin, accumulation of halfmer polysomes, and reduced levels of 60S ribosomal subunits .

• Localization: Nip7p is found in both the cytoplasm and nucleus, with a significant amount localized in the nucleolus .

Potential Applications

While specific research on the Tetraodon nigroviridis NIP7 homolog is limited, studies on its homologs suggest potential applications in understanding ribosome biogenesis and its implications for cellular health and disease.

Data Tables

Given the lack of specific data on the Tetraodon nigroviridis NIP7 homolog, we can consider general information about NIP7 proteins and their functions:

References Antibodies-online.com. (2019). NIP7 Protein (AA 1-180) (Strep Tag). PubMed Central. (1997). Saccharomyces cerevisiae Nip7p is required for efficient 60S ribosome subunit biogenesis. PubMed Central. (2011). The G protein-coupled receptors in the pufferfish Takifugu rubripes. PubMed. (2022). Saccharomyces cerevisiae Nip7p is required for efficient 60S ribosome subunit biogenesis. Antibodies-online.com. NIP7 Proteins. Frontiers in Marine Science. (2022). Multi-Omics Study on the Molecular Mechanisms of Tetraodon Nigroviridis Resistance to Exogenous Vibrio Parahaemolyticus Infection. UniProt. Tetraodon nigroviridis (Spotted green pufferfish). PubMed Central. (2021). Lead Optimization of 3,5-Disubstituted-7-Azaindoles for the Treatment of Human African Trypanosomiasis.