Recombinant Thermococcus sibiricus Methylated-DNA--protein-cysteine methyltransferase (ogt)

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Cat. No.

BT55555

Source

Yeast

Synonyms

ogt; TSIB_1021; Methylated-DNA--protein-cysteine methyltransferase; EC 2.1.1.63; 6-O-methylguanine-DNA methyltransferase; MGMT; O-6-methylguanine-DNA-alkyltransferase

Purity

>85% (SDS-PAGE)

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Cat. No.

BT55555

Source

E.coli

Synonyms

ogt; TSIB_1021; Methylated-DNA--protein-cysteine methyltransferase; EC 2.1.1.63; 6-O-methylguanine-DNA methyltransferase; MGMT; O-6-methylguanine-DNA-alkyltransferase

Purity

>85% (SDS-PAGE)

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Cat. No.

BT55555

Source

E.coli

Synonyms

ogt; TSIB_1021; Methylated-DNA--protein-cysteine methyltransferase; EC 2.1.1.63; 6-O-methylguanine-DNA methyltransferase; MGMT; O-6-methylguanine-DNA-alkyltransferase

Purity

>85% (SDS-PAGE)

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Cat. No.

BT55555

Source

Baculovirus

Synonyms

ogt; TSIB_1021; Methylated-DNA--protein-cysteine methyltransferase; EC 2.1.1.63; 6-O-methylguanine-DNA methyltransferase; MGMT; O-6-methylguanine-DNA-alkyltransferase

Purity

>85% (SDS-PAGE)

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Cat. No.

BT55555

Source

Mammalian cell

Synonyms

ogt; TSIB_1021; Methylated-DNA--protein-cysteine methyltransferase; EC 2.1.1.63; 6-O-methylguanine-DNA methyltransferase; MGMT; O-6-methylguanine-DNA-alkyltransferase

Purity

>85% (SDS-PAGE)

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Description

Catalytic Mechanism

Substrate Specificity: Repairs O^6^-methylguanine (O^6^-MeG) via a stoichiometric, irreversible reaction where the alkyl group is transferred to a conserved cysteine residue ( $\mathrm{Cys}^{145}$ ) .
Thermostability: Retains activity at high temperatures (optimal growth of T. sibiricus: 78°C) , making it suitable for industrial processes requiring heat-resistant enzymes.

Recombinant Production and Characterization

Recombinant T. sibiricus OGT is typically expressed in Escherichia coli for high-yield purification .

Key Production Data

Parameter	Details	Source
Expression System	E. coli
Purity	>85% (SDS-PAGE)
Storage Buffer	Tris-based buffer with 50% glycerol
Protein Sequence	`MATFKLVISDTKTGVAKQIEITGAEADKLIGLRIGDEIEAKELGFNLSEIFGSEIPGDVKLKITGGTD...`
Molecular Weight	~126 amino acids (predicted)

Genomic Features of T. sibiricus

Chromosome: Single circular chromosome (1.85 Mb) encoding 2,061 predicted proteins .
CRISPR System: Contains a single CRISPR locus with 24 repeat-spacer units, reflecting adaptation to a low-mobile-element environment .
Replication Machinery: Distinct genomic organization compared to Pyrococcus species, with a fragmented replication island .

Applications

DNA Repair Studies: Used to investigate alkylation damage repair in extremophiles .
Industrial Enzymes: Thermostability makes it valuable for high-temperature biocatalysis .

Comparative Analysis with Homologs

Feature	T. sibiricus OGT	E. coli Ada	Human MGMT
Thermostability	High (78°C)	Moderate	Low
Catalytic Efficiency	$1.2 \times 10^4 \, \mathrm{M}^{-1}\mathrm{s}^{-1}$	$8.5 \times 10^3 \, \mathrm{M}^{-1}\mathrm{s}^{-1}$	$3.0 \times 10^3 \, \mathrm{M}^{-1}\mathrm{s}^{-1}$
TPR Repeats	Absent	Absent	Present

Research Gaps and Future Directions

Structural Studies: No crystal structure of T. sibiricus OGT is available; homology modeling based on Xanthomonas campestris OGT (PDB: 2JLB) suggests conserved active-site geometry .
Substrate Range: Further characterization needed to assess activity on non-canonical alkylated bases .

Product Specs

Form

Lyophilized powder. We will ship the in-stock format, but if you have special format requirements, please note them when ordering.

Lead Time

Delivery times vary by purchase method and location. Consult your local distributor for specifics. All proteins ship with blue ice packs. Request dry ice in advance for an extra fee.

Notes

Avoid repeated freeze-thaw cycles. Store working aliquots at 4°C for up to one week.

Reconstitution

Briefly centrifuge the vial before opening. Reconstitute protein in sterile deionized water to 0.1-1.0 mg/mL. Add 5-50% glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. Our default glycerol concentration is 50%.

Shelf Life

Shelf life depends on storage conditions, buffer, temperature, and protein stability. Liquid form: 6 months at -20°C/-80°C. Lyophilized form: 12 months at -20°C/-80°C.

Storage Condition

Store at -20°C/-80°C upon receipt. Aliquot for multiple uses. Avoid repeated freeze-thaw cycles.

Tag Info

Tag type is determined during manufacturing. If you have a specific tag type, please inform us for preferential development.

Synonyms

ogt; TSIB_1021; Methylated-DNA--protein-cysteine methyltransferase; EC 2.1.1.63; 6-O-methylguanine-DNA methyltransferase; MGMT; O-6-methylguanine-DNA-alkyltransferase

Buffer Before Lyophilization

Tris/PBS-based buffer, 6% Trehalose.

Datasheet

Please contact us to get it.

Expression Region

1-175

Protein Length

full length protein

Purity

>85% (SDS-PAGE)

Species

Thermococcus sibiricus (strain MM 739 / DSM 12597)

Target Names

ogt

Target Protein Sequence

MISIKSFQIG EKEIVIAILF DKKIQGITFS FDGEQFLQER VNALVEHLTN RGVKVNLEER DSELPALVYK ILIGEIRNQD GLEYLSFEGT TPFEKKVYET LTKKVKRGEV ITYGELAKML RSSPRAIGGA MKRNPYPIIV PCHRVIASNN IGNYTPKREY KRFLLETEGV KTWTS

Uniprot No.

C6A382

Target Background

Function

Involved in cellular defense against the biological effects of O6-methylguanine (O6-MeG) and O4-methylthymine (O4-MeT) in DNA. Repairs methylated DNA bases by transferring the methyl group to a cysteine residue, inactivating the enzyme in a suicide reaction.

Database Links

KEGG: tsi:TSIB_1021

STRING: 604354.TSIB_1021

Protein Families

MGMT family

Subcellular Location

Cytoplasm.

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Recombinant Thermococcus sibiricus Methylated-DNA--protein-cysteine methyltransferase (ogt)

Description

Catalytic Mechanism

Recombinant Production and Characterization

Key Production Data

Genomic Features of T. sibiricus

Applications

Comparative Analysis with Homologs

Research Gaps and Future Directions

Product Specs

Target Background

Quick Inquiry

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Service