Recombinant Thermomicrobium roseum ATP synthase subunit alpha (atpA), partial

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Cat. No.
BT39995
Source
Yeast
Synonyms
atpA; trd_1230; ATP synthase subunit alpha; EC 7.1.2.2; ATP synthase F1 sector subunit alpha; F-ATPase subunit alpha
Purity
>85% (SDS-PAGE)
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Cat. No.
BT39995
Source
E.coli
Synonyms
atpA; trd_1230; ATP synthase subunit alpha; EC 7.1.2.2; ATP synthase F1 sector subunit alpha; F-ATPase subunit alpha
Purity
>85% (SDS-PAGE)
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Cat. No.
BT39995
Source
E.coli
Synonyms
atpA; trd_1230; ATP synthase subunit alpha; EC 7.1.2.2; ATP synthase F1 sector subunit alpha; F-ATPase subunit alpha
Purity
>85% (SDS-PAGE)
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Cat. No.
BT39995
Source
Baculovirus
Synonyms
atpA; trd_1230; ATP synthase subunit alpha; EC 7.1.2.2; ATP synthase F1 sector subunit alpha; F-ATPase subunit alpha
Purity
>85% (SDS-PAGE)
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Cat. No.
BT39995
Source
Mammalian cell
Synonyms
atpA; trd_1230; ATP synthase subunit alpha; EC 7.1.2.2; ATP synthase F1 sector subunit alpha; F-ATPase subunit alpha
Purity
>85% (SDS-PAGE)
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Description

Biological Role of ATP Synthase Subunit Alpha

The α-subunit (encoded by atpA) is a core component of the F1 sector of ATP synthase, responsible for catalytic activity. It binds nucleotides and works cooperatively with the β-subunit to drive ATP synthesis via rotational catalysis . In T. roseum, this enzyme operates under extreme thermophilic conditions (70–75°C), necessitating structural stability .

Genomic Context of atpA in Thermomicrobium roseum

The T. roseum genome (DSM 5159) was fully sequenced, revealing its placement within the Chloroflexi phylum . Key genomic features related to atpA include:

FeatureDetail
Genome size~2.2 Mbp
atpA operon organizationLikely clustered with other ATP synthase genes (e.g., atpB, atpG)
Co-regulated genesRibosomal pseudouridine synthase, membrane transporters
Regulatory elementsPalindromic promoter motifs (e.g., tcctcccCtGgtCgGatcttcg) upstream

The atpA gene is hypothesized to be part of a conserved operon interrupted by auxiliary genes like phenazine biosynthesis proteins .

Evolutionary and Phylogenetic Significance

  • atpA sequences provide higher phylogenetic resolution than 16S rRNA, differentiating closely related species with >89% sequence similarity .

  • In T. roseum, atpA phylogeny supports its classification within Chloroflexi, resolving past ambiguities from rRNA-based trees .

Biotechnological Potential

  • Thermostable Enzymes: Partial atpA fragments could be used to engineer heat-stable ATP synthases for industrial biocatalysis .

  • Metabolic Engineering: ATP synthase modifications might enhance ATP yield in biofuel-producing strains .

Unresolved Questions

  • Regulatory Mechanisms: The role of upstream motifs (e.g., tcctcccCtGgtCgGatcttcg) in atpA expression remains unverified experimentally .

  • Structural Data: No crystal structures exist for T. roseum ATP synthase; computational models are needed to infer partial recombinant protein behavior.

Product Specs

Form
Lyophilized powder. We will ship the available format, but if you have special format requirements, please specify them when ordering.
Lead Time
Delivery time varies by purchase method and location. Consult local distributors for specifics. Proteins are shipped with blue ice packs; dry ice requires prior arrangement and extra fees.
Notes
Avoid repeated freeze-thaw cycles. Working aliquots can be stored at 4°C for up to one week.
Reconstitution
Briefly centrifuge the vial before opening. Reconstitute in sterile deionized water to 0.1-1.0 mg/mL. Add 5-50% glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. Our default final glycerol concentration is 50%.
Shelf Life
Shelf life depends on storage conditions, buffer, temperature, and protein stability. Liquid form: 6 months at -20°C/-80°C. Lyophilized form: 12 months at -20°C/-80°C.
Storage Condition
Store at -20°C/-80°C upon arrival. Aliquot for multiple uses. Avoid repeated freeze-thaw cycles.
Tag Info
Tag type is determined during manufacturing. If you require a specific tag, please inform us and we will prioritize its development.
Synonyms
atpA; trd_1230; ATP synthase subunit alpha; EC 7.1.2.2; ATP synthase F1 sector subunit alpha; F-ATPase subunit alpha
Buffer Before Lyophilization
Tris/PBS-based buffer, 6% Trehalose.
Datasheet
Please contact us to get it.
Protein Length
Partial
Purity
>85% (SDS-PAGE)
Species
Thermomicrobium roseum (strain ATCC 27502 / DSM 5159 / P-2)
Target Names
atpA
Uniprot No.
B9L1H1

Target Background

Function
Generates ATP from ADP using a proton gradient across the membrane. The alpha chain is regulatory.
Database Links

KEGG: tro:trd_1230

STRING: 309801.trd_1230

Protein Families
ATPase alpha/beta chains family
Subcellular Location
Cell membrane; Peripheral membrane protein.

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